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oai:repisalud.isciii.es:20.500.12105/201672024-11-28T21:12:04Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Nadal-Serrano, Mercedes author Sastre-Serra, Jorge author Valle, Adamo author Roca, Pilar author Oliver, Jordi author 2015 Background/Aims: Large-scale epidemiological studies support a correlation between obesity and breast cancer in postmenopausal women. Circulating leptin levels are increased in obese and it has been suggested to play a significant role in mammary tumor formation and progression. Moreover, regulation of oxidative stress is another important factor in both tumor development and responses to anticancer therapies. The aim of this study was to examine the relationship between oxidative stress and chronic leptin exposure. Methods: We treated MCF-7 breast cancer cells with 100 ng/mL leptin for 10 days and analyzed cell growth, ROS production and oxidative damage, as well as, some of the main antioxidant systems. Furthermore, since the hyperleptinemia has been associated with a worse pathology prognosis, we decided to test the influence of leptin in response to cisplatin anticancer treatment. Results: Leptin signalling increased cell proliferation but reduced ROS production, as well as, oxidative damage. We observed an upregulation of SIRT1 after leptin exposure, a key regulator of stress response and metabolism. Additionally, leptin counteracted cisplatin-induced cytotoxicity in tumor cells, showing a decrease in cell death. Conclusion: Chronic leptin could contribute to the effective regulation of endogenous and treatment-induced oxidative stress, and it contributes to explain in part its proliferative effects. Nadal Serrano M, Sastre-Serra J, Valle A, Roca P, Oliver Oliver J. Chronic-Leptin Attenuates Cisplatin Cytotoxicity in MCF-7 Breast Cancer Cell Line. Cell Physiol Biochem. 2015;36(1):221-32. Epub 2015 Apr 30. 10.1159/000374066 1421-9778 1015-8987 Cellular Physiology and Biochemistry http://hdl.handle.net/20.500.13003/11705 25967962 L604539089 2-s2.0-84929908549 http://hdl.handle.net/20.500.12105/20167 355017300018 Oxidative stress Leptin Breast cancer Cell growth Sirtuin1 Cisplatin drug Chronic-Leptin Attenuates Cisplatin Cytotoxicity in MCF-7 Breast Cancer Cell Line