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oai:repisalud.isciii.es:20.500.12105/201272024-09-22T06:01:55Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967

00925njm 22002777a 4500 dc Tomas, Anna author Lery, Leticia author Regueiro, Veronica author Perez-Gutierrez, Camino author Martinez, Veronica author Moranta, David author Llobet, Enrique author González-Nicolau, María Del Mar author Insua, Jose L author Tomas, Juan M author Sansonetti, Philippe J author Tournebize, Regis author Bengoechea, Jose Antonio author 2015-07-03 Klebsiella pneumoniae is an etiologic agent of community-acquired and nosocomial pneumonia. It has been shown that K. pneumoniae infections are characterized by reduced early inflammatory response. Recently our group has shown that K. pneumoniae dampens the activation of inflammatory responses by antagonizing the activation of the NF-kappa B canonical pathway. Our results revealed that K. pneumoniae capsule polysaccharide (CPS) was necessary but not sufficient to attenuate inflammation. To identify additional Klebsiella factors required to dampen inflammation, we standardized and applied a high-throughput gain-of-function screen to examine a Klebsiella transposon mutant library. We identified 114 mutants that triggered the activation of NF-kappa B. Two gene ontology categories accounted for half of the loci identified in the screening: metabolism and transport genes (32% of the mutants) and envelope-related genes (17%). Characterization of the mutants revealed that the lack of the enterobactin siderophore was linked to a reduced CPS expression, which in turn underlined the NF-kappa B activation induced by the mutant. The lipopolysaccharide (LPS) O-polysaccharide and the pullulanase (PulA) type 2 secretion system (T2SS) are required for full effectiveness of the immune evasion. Importantly, these factors do not play a redundant role. The fact that LPS O-polysaccharide and T2SS mutant-induced responses were dependent on TLR2-TLR4-MyD88 activation suggested that LPS O-polysaccharide and PulA perturbed Toll-like receptor (TLR)-dependent recognition of K. pneumoniae. Finally, we demonstrate that LPS O-polysaccharide and pulA mutants are attenuated in the pneumonia mouse model. We propose that LPS O-polysaccharide and PulA T2SS could be new targets for the design of new antimicrobials. Increasing TLR-governed defense responses might provide also selective alternatives for the management of K. pneumoniae pneumonia. Tomás Sangenis A, Lery L, Regueiro Veronica, Perez-Gutierrez Camino, Martinez Veronica, Moranta D, et al. Functional Genomic Screen Identifies Klebsiella pneumoniae Factors Implicated in Blocking Nuclear Factor kappa B (NF-kappa B) Signaling. J Biol Chem. 2015 Jul 03;290(27):16678-97. Epub 2015 May 13. 10.1074/jbc.M114.621292 1083-351X Journal of Biological Chemistry http://hdl.handle.net/20.500.13003/10779 25971969 L605101850 2-s2.0-84936761384 http://hdl.handle.net/20.500.12105/20127 357572800018 Functional Genomic Screen Identifies Klebsiella pneumoniae Factors Implicated in Blocking Nuclear Factor kappa B (NF-kappa B) Signaling