2026-07-22T05:26:14Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/200272025-04-04T09:27:33Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud author Caballero, Juan de Dios author del Campo, Rosa author Tato, Marta author Gomez Garcia de la Pedrosa, Elia author Cobo, Marta author López-Causapé, Carla author Gomez-Mampaso, Enrique author Oliver, Antonio author Canton, Rafael author Spanish Network Cystic Fibrosis Mi 2024-07-03T11:09:41Z 2024-07-03T11:09:41Z 2014-12-24 De Dios Caballero J, Del Campo R, Tato M, Gomez G De La Pedrosa E, Cobo M, López-Causapé C, et al. Microbiological diagnostic procedures for respiratory cystic fibrosis samples in Spain: towards standard of care practices. BMC Microbiol. 2014 Dec 24;14:335. 10.1186/s12866-014-0335-y 1471-2180 BMC Microbiology http://hdl.handle.net/20.500.13003/11037 25927861 L602614249 2-s2.0-84923874273 http://hdl.handle.net/20.500.12105/20027 348183300001 Background: The microbiological procedures for cystic fibrosis (CF) samples of 17 participating Spanish centers were examined to verify their compliance with current international and national guidelines and to implement the best standards of care for microbiology practices. A 47-item questionnaire covering different CF microbiology aspects was sent to participant laboratories. Telephone interviews were performed when necessary. Data about samples processing for bacteria, mycobacteria and fungi were collected. Results: Gene sequencing (71%), MALDI-TOF (59%) or both (94%) were available for most laboratories. Susceptibility testing was performed by automated microdilution systems (94%) and manual diffusion methods (59%). However, a low use of selective media for Staphylococcus aureus (59%) and Burkholderia cepacia complex (71%), and of epidemiological typing methods (41%) was reported. Conclusions: Most Spanish laboratories are in agreement with consensus guidelines for the processing of CF respiratory samples, but need to improve in the use of specific selective media and typing methods for epidemiologic studies. eng Cystic fibrosis Microbiology Laboratory procedures Respiratory samples Consensus guidelines Microbiological diagnostic procedures for respiratory cystic fibrosis samples in Spain: towards standard of care practices research article