2026-06-14T03:32:03Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/199592024-11-28T19:55:49Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16967
Repisalud
author
Maria Torrens, Juana
author
Orellana-Gavalda, Josep M
author
Palou, Mariona
author
Sanchez, Juana
author
Herrero, Laura
author
Picó, Catalina
author
Serra, Dolors
author
Palou, Andreu
2024-07-03T11:01:18Z
2024-07-03T11:01:18Z
2014
Maria Torrens J, Orellana-Gavalda JM, Palou March M, Sánchez J, Herrero L, Pico C, et al. Enhancing Hepatic Fatty Acid Oxidation as a Strategy for Reversing Metabolic Disorders Programmed by Maternal Undernutrition During Gestation. Cell Physiol Biochem. 2014;33(5):1498-515. Epub 2014 May 14.
10.1159/000358714
1421-9778
1015-8987
Cellular Physiology and Biochemistry
http://hdl.handle.net/20.500.13003/11863
24854839
L373159366
2-s2.0-84901334432
http://hdl.handle.net/20.500.12105/19959
336493300022
Background/Aims: Moderate maternal calorie-restriction during gestation programmes offspring for a major propensity to develop metabolic alterations in adulthood. We aimed to assess whether increased hepatic fatty-acid oxidation (FAO), at early ages, by gene transfer of Cpt1am (active mutant of carnitine palmitoyltransferase-1a), may be a strategy for reversing metabolic disturbances associated to maternal calorie-restriction during gestation in rats. Methods: AAV-Gfp (control) and AAV-Cpt1am vectors were administered by tail vein injection in 18-day-old control-pups and the offspring of 20% calorie-restricted rats during gestation (CR). After weaning, animals were fed with normal-fat diet. At the age of 4 months, they were moved to HF-diet and sacrificed at the age of 6 months to collect tissues. Locomotive activity, energy expenditure and blood pressure were measured. Results: Under HF-diet, CR-animals showed higher HOMA-IR, adipocyte diameter and hepatic triglyceride accumulation than controls; these alterations were reverted in Cpt1am-injected animals. In liver, this treatment ameliorated inflammatory state, decreased expression of lipogenesis-related genes and partially restored the decreased expression of leptin-receptor occurring in CR-animals. Treatment also reverted the decreased energy expenditure and the increased blood pressure of CR-animals. Conclusion: Increasing hepatic FAO through AAV-Cpt1am injection at juvenile ages prevents some metabolic disorders associated to gestational maternal calorie-restriction.
eng
Cpt1a
Calorie restriction
Gestation
Gene transference
Liver
Enhancing Hepatic Fatty Acid Oxidation as a Strategy for Reversing Metabolic Disorders Programmed by Maternal Undernutrition During Gestation
research article