2026-04-29T12:23:04Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/199312024-09-27T09:54:40Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Rodríguez, Diego
author
Moscoso, Miguel
author
Desco, Manuel
author
Ripoll, Jorge
author
Fernández, Roberto
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación ProCNIC
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2024-07-03T09:54:53Z
2024-07-03T09:54:53Z
2024-04-25
Sci Rep. 2024 Apr 25;14(1):9536.
10.1038/s41598-024-60158-8
2045-2322
Scientific reports
38664522
http://hdl.handle.net/20.500.12105/19931
Neocaridina davidi, a small freshwater shrimp native to Asia, specifically China, Japan, Korea, and Vietnam, possesses remarkable resistance to poor water quality and offers various advantages over other invertebrate species to examine crucial issues in neuroscience and other related areas. These advantages include robustness, ease of maintenance, and transparency, making them useful for in vivo studies with optical imaging techniques. Despite its suitability for research purposes, particularly in the fields of imaging and fluorescent techniques, the lack of attention given to this species has resulted in the absence of a robust and replicable sedation protocol for immobilization and safe manipulation. Consequently, researchers face challenges in performing experimental procedures while minimizing harm to this specimen. In this study, we have developed and evaluated a simple sedation protocol specifically designed for Neocaridina davidi, assessing its effectiveness using light microscopy and image processing.
eng
Development and testing of a sedation protocol for Neocaridina davidi.
journal article
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URL
https://repisalud.isciii.es/bitstreams/3d013499-64c9-48d4-810d-43f9ec465fad/download
File
MD5
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application/pdf
Development and testing of a sedation_Sci Rep_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/db56681e-82b9-43f3-90a9-d11fb9a9f4c5/download
File
MD5
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text/plain
Development and testing of a sedation_Sci Rep_2024.pdf.txt