2026-04-29T12:23:44Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/199312024-09-27T09:54:40Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Rodríguez, Diego author Moscoso, Miguel author Desco, Manuel author Ripoll, Jorge author Fernández, Roberto author 2024-04-25 Neocaridina davidi, a small freshwater shrimp native to Asia, specifically China, Japan, Korea, and Vietnam, possesses remarkable resistance to poor water quality and offers various advantages over other invertebrate species to examine crucial issues in neuroscience and other related areas. These advantages include robustness, ease of maintenance, and transparency, making them useful for in vivo studies with optical imaging techniques. Despite its suitability for research purposes, particularly in the fields of imaging and fluorescent techniques, the lack of attention given to this species has resulted in the absence of a robust and replicable sedation protocol for immobilization and safe manipulation. Consequently, researchers face challenges in performing experimental procedures while minimizing harm to this specimen. In this study, we have developed and evaluated a simple sedation protocol specifically designed for Neocaridina davidi, assessing its effectiveness using light microscopy and image processing. Sci Rep. 2024 Apr 25;14(1):9536. 10.1038/s41598-024-60158-8 2045-2322 Scientific reports 38664522 http://hdl.handle.net/20.500.12105/19931 Development and testing of a sedation protocol for Neocaridina davidi.