2026-04-29T04:09:33Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/199112024-09-27T08:55:20Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19605col_20.500.12105_16977col_20.500.12105_16978
Repisalud
author
González, María Isabel
author
González-Arjona, Mario
author
Cusso, Lorena
author
Morcillo, Miguel Ángel
author
Aguilera-Correa, John Jairo
author
Esteban, Jaime
author
Kestler, Martha
author
Calle, Daniel
author
Cerón, Carlos
author
Cortes-Canteli, Marta
author
Muñoz, Patricia
author
Bouza, Emilio
author
Desco, Manuel
author
Salinas, Beatriz
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación Ramón Areces
funder
Comunidad de Madrid (España)
funder
Fundación BBVA
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación ProCNIC
2024-07-02T13:03:21Z
2024-07-02T13:03:21Z
2024
Int J Biomed Imaging. 2024 Apr 18:2024:3655327
1687-4188
http://hdl.handle.net/20.500.12105/19911
38665417
10.1155/2024/3655327
International journal of biomedical imaging
PURPOSE
The Gram-positive Staphylococcus aureus bacterium is one of the leading causes of infection in humans. The lack of specific noninvasive techniques for diagnosis of staphylococcal infection together with the severity of its associated complications support the need for new specific and selective diagnostic tools. This work presents the successful synthesis of an immunotracer that targets the α-toxin released by S. aureus.
METHODS
[89Zr]Zr-DFO-ToxAb was synthesized based on radiolabeling an anti-α-toxin antibody with zirconium-89. The physicochemical characterization of the immunotracer was performed by high-performance liquid chromatography (HPLC), radio-thin layer chromatography (radio-TLC), and electrophoretic analysis. Its diagnostic ability was evaluated in vivo by positron emission tomography/computed tomography (PET/CT) imaging in an animal model of local infection-inflammation (active S. aureus vs. heat-killed S. aureus) and infective osteoarthritis.
RESULTS
Chemical characterization of the tracer established the high radiochemical yield and purity of the tracer while maintaining antibody integrity. In vivo PET/CT image confirmed the ability of the tracer to detect active foci of S. aureus. Those results were supported by ex vivo biodistribution studies, autoradiography, and histology, which confirmed the ability of [89Zr]Zr-DFO-ToxAb to detect staphylococcal infectious foci, avoiding false-positives derived from inflammatory processes.
CONCLUSIONS
We have developed an immuno-PET tracer capable of detecting S. aureus infections based on a radiolabeled antibody specific for the staphylococcal alpha toxins. The in vivo assessment of [89Zr]Zr-DFO-ToxAb confirmed its ability to selectively detect staphylococcal infectious foci, allowing us to discern between infectious and inflammatory processes.
eng
In Vivo Detection of Staphylococcus aureus Infections Using Radiolabeled Antibodies Specific for Bacterial Toxins.
journal article
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In Vivo Detection of Staphylococcus aureus_Int J Biomed Imaging_2024.pdf
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In Vivo Detection of Staphylococcus aureus_Int J Biomed Imaging_2024.pdf.txt