2026-04-29T23:04:18Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/199072024-09-21T12:21:55Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Ugalde-Triviño, Lola
author
Molina-Jiménez, Francisca
author
H-Vázquez, Juan
author
Relaño-Rupérez, Carlos
author
Arias-González, Laura
author
Casabona, Sergio
author
Pérez-Fernández, María Teresa
author
Martín-Domínguez, Verónica
author
Fernández-Pacheco, Jennifer
author
Lucendo, Alfredo J
author
Bernardo, David
author
Santander, Cecilio
author
Majano, Pedro
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Ciencia (España)
funder
Unión Europea. Comisión Europea. NextGenerationEU
funder
Comunidad de Madrid (España)
2024-07-02T12:10:40Z
2024-07-02T12:10:40Z
2024
Front Immunol. 2024 Apr 5:15:1374611.
10.3389/fimmu.2024.1374611
1664-3224
Frontiers in immunology
38646544
http://hdl.handle.net/20.500.12105/19907
OBJECTIVES
The aim of the study was to characterize the circulating immunome of patients with EoE before and after proton pump inhibitor (PPI) treatment in order to identify potential non-invasive biomarkers of treatment response.
METHODS
PBMCs from 19 healthy controls and 24 EoE patients were studied using a 39-plex spectral cytometry panel. The plasmacytoid dendritic cell (pDC) population was differentially characterized by spectral cytometry analysis and immunofluorescence assays in esophageal biopsies from 7 healthy controls and 13 EoE patients.
RESULTS
Interestingly, EoE patients at baseline had lower levels of circulating pDC compared with controls. Before treatment, patients with EoE who responded to PPI therapy had higher levels of circulating pDC and classical monocytes, compared with non-responders. Moreover, following PPI therapy pDC levels were increased in all EoE patients, while normal levels were only restored in PPI-responding patients. Finally, circulating pDC levels inversely correlated with peak eosinophil count and pDC count in esophageal biopsies. The number of tissue pDCs significantly increased during active EoE, being even higher in non-responder patients when compared to responder patients pre-PPI. pDC levels decreased after PPI intake, being further restored almost to control levels in responder patients post-PPI.
CONCLUSIONS
We hereby describe a unique immune fingerprint of EoE patients at diagnosis. Moreover, circulating pDC may be also used as a novel non-invasive biomarker to predict subsequent response to PPI treatment.
eng
Circulating immunome fingerprint in eosinophilic esophagitis is associated with clinical response to proton pump inhibitor treatment.
journal article
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Circulating immunome fingerprint_Front Immunol_2024.pdf
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https://repisalud.isciii.es/bitstreams/617f0f61-8a9b-4a8e-9235-501f970d040b/download
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text/plain
Circulating immunome fingerprint_Front Immunol_2024.pdf.txt