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oai:repisalud.isciii.es:20.500.12105/196902024-11-28T16:48:48Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16958col_20.500.12105_19617

00925njm 22002777a 4500 dc Jiménez-Hidalgo, María author Kurz, Cyril Léopold author Pedrajas, José Rafael author Naranjo-Galindo, Francisco José author González-Barrios, María author Cabello, Juan author Saez, Alberto G author Lozano, Encarnacion author Button, Emma L author Veal, Elizabeth A author Fierro-González, Juan Carlos author Swoboda, Peter author Miranda-Vizuete, Antonio author 2014-03 Thioredoxins are a class of evolutionarily conserved proteins that have been demonstrated to play a key role in many cellular processes involving redox reactions. We report here the genetic and biochemical characterization of Caenorhabditis elegans TRX-3, the first metazoan thioredoxin with an intestine-specific expression pattern. By using green fluorescent protein reporters we have found that TRX-3 is expressed in both the cytoplasm and the nucleus of intestinal cells, with a prominent localization at the apical membrane. Although intestinal function, reproductive capacity, longevity, and resistance of trx-3 loss-of-function mutants to many stresses are indistinguishable from those of wild-type animals, we have observed a slight reduction in size and a minor reduction in the defecation cycle timing of trx-3 mutants. Interestingly, trx-3 is induced upon infection by Photorhabdus luminescens and Candida albicans, and TRX-3 overexpression provides a modest protection against these pathogens. Together, our data indicate that TRX-3 function in the intestine is dispensable for C. elegans development but may be important to fight specific bacterial and fungal infections. Free Radic Biol Med. 2014 Mar;68(100):205-19. 10.1016/j.freeradbiomed.2013.11.023 1873-4596 Free radical biology & medicine 24316195 http://hdl.handle.net/20.500.12105/19690 Caenorhabditis elegans Thioredoxin Intestine Stress Pathogen infection Photorhabdus luminescens Candida albicans Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin