2026-04-29T23:03:55Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/195212024-09-27T08:09:05Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Li-Bao, Lin
author
Díaz-Díaz, Covadonga
author
Raiola, Morena
author
Sierra, Rocío
author
Temiño, Susana
author
Moya, Francisco J
author
Rodriguez-Perales, Sandra
author
Santos, Elisa
author
Giovinazzo, Giovanna
author
Bleckwehl, Tore
author
Rada-Iglesias, Álvaro
author
Spitz, Francois
author
Torres, Miguel
funder
Agencia Estatal de Investigación (España)
funder
Unión Europea. Comisión Europea
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Fundación ProCNIC
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2024-05-22T10:05:21Z
2024-05-22T10:05:21Z
2024-05-10
Nat Commun. 2024 May 10;15(1):3931
10.1038/s41467-024-48258-5
2041-1723
Nature communications
38729993
http://hdl.handle.net/20.500.12105/19521
MYC plays various roles in pluripotent stem cells, including the promotion of somatic cell reprogramming to pluripotency, the regulation of cell competition and the control of embryonic diapause. However, how Myc expression is regulated in this context remains unknown. The Myc gene lies within a ~ 3-megabase gene desert with multiple cis-regulatory elements. Here we use genomic rearrangements, transgenesis and targeted mutation to analyse Myc regulation in early mouse embryos and pluripotent stem cells. We identify a topologically-associated region that homes enhancers dedicated to Myc transcriptional regulation in stem cells of the pre-implantation and early post-implantation embryo. Within this region, we identify elements exclusively dedicated to Myc regulation in pluripotent cells, with distinct enhancers that sequentially activate during naive and formative pluripotency. Deletion of pluripotency-specific enhancers dampens embryonic stem cell competitive ability. These results identify a topologically defined enhancer cluster dedicated to early embryonic expression and uncover a modular mechanism for the regulation of Myc expression in different states of pluripotency.
eng
Regulation of Myc transcription by an enhancer cluster dedicated to pluripotency and early embryonic expression.
journal article
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URL
https://repisalud.isciii.es/bitstreams/7946d0dd-52a1-4a09-a0e0-d6f33c8c8ca4/download
File
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application/pdf
Regulation of Myc transcription_Nat Commun_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/0917776f-368b-42c5-8410-0eeab1d6d966/download
File
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text/plain
Regulation of Myc transcription_Nat Commun_2024.pdf.txt