2026-04-29T23:03:52Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/195212024-09-27T08:09:05Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Li-Bao, Lin author Díaz-Díaz, Covadonga author Raiola, Morena author Sierra, Rocío author Temiño, Susana author Moya, Francisco J author Rodriguez-Perales, Sandra author Santos, Elisa author Giovinazzo, Giovanna author Bleckwehl, Tore author Rada-Iglesias, Álvaro author Spitz, Francois author Torres, Miguel author 2024-05-10 MYC plays various roles in pluripotent stem cells, including the promotion of somatic cell reprogramming to pluripotency, the regulation of cell competition and the control of embryonic diapause. However, how Myc expression is regulated in this context remains unknown. The Myc gene lies within a ~ 3-megabase gene desert with multiple cis-regulatory elements. Here we use genomic rearrangements, transgenesis and targeted mutation to analyse Myc regulation in early mouse embryos and pluripotent stem cells. We identify a topologically-associated region that homes enhancers dedicated to Myc transcriptional regulation in stem cells of the pre-implantation and early post-implantation embryo. Within this region, we identify elements exclusively dedicated to Myc regulation in pluripotent cells, with distinct enhancers that sequentially activate during naive and formative pluripotency. Deletion of pluripotency-specific enhancers dampens embryonic stem cell competitive ability. These results identify a topologically defined enhancer cluster dedicated to early embryonic expression and uncover a modular mechanism for the regulation of Myc expression in different states of pluripotency. Nat Commun. 2024 May 10;15(1):3931 10.1038/s41467-024-48258-5 2041-1723 Nature communications 38729993 http://hdl.handle.net/20.500.12105/19521 Regulation of Myc transcription by an enhancer cluster dedicated to pluripotency and early embryonic expression.