2026-06-14T03:46:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/194422024-09-22T20:21:59Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19613
Repisalud
author
Pinos, Helena
author
Sánchez-Serrano, Ricardo
author
Carrillo, Beatriz
author
Fernández-García, José Manuel
author
García-Úbeda, Rocío
author
de Paz, Ana
author
López-Tolsa, Gabriela E
author
Vidal, Pedro
author
Gutiérrez-Ferre, Valeria
author
Pellón, Ricardo
author
Collado, Paloma
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Banco Santander
funder
National University of Distance Education (España)
2024-05-17T09:51:32Z
2024-05-17T09:51:32Z
2023-01-05
Behav Brain Res. 2023 Jan 5:436:114055.
10.1016/j.bbr.2022.114055
1872-7549
Behavioural brain research
35964782
http://hdl.handle.net/20.500.12105/19442
The objective of this study was to investigate the orexin and POMC populations in the hypothalamic nuclei of male Wistar rats after the activity-based anorexia (ABA) procedure. Four groups were established based on food restriction and activity: activity (A), ABA, diet (D) and control (C). The ABA protocol consisted of free access to a running wheel for a period of 22 h and access to food for 1 h. When the animals in the ABA group reached the ABA criterion, were sacrificed, and their brains were collected and serially sectioned. The free-floating sections were processed for orexin and POMC immunostaining. The number of orexin A-ir cells in the perifornical-dorsomedial-hypothalamus continuum (PFD) and lateral hypothalamus (LH) and the number of POMC-ir cells in the arcuate nucleus (Arc) were estimated. Data on food intake, body weight and wheel turns were also analyzed. The ABA procedure caused a significant decrease in body weight along with a significant increase in activity. Moreover, at the end of the ABA procedure, the number of POMC-ir cells decreased in the Arc in the A group, and significantly more in the ABA group, and the number of orexin A-ir positive cells decreased in the LH in D and ABA groups. The differential decrease in POMC in the ABA group emphasizes the importance of the melanocortin system in the maintenance of ABA, but more research is needed to elucidate the involvement of this peptide in the mechanism that promotes and maintains anorexia nervosa and how increased activity may interact with all these processes.
eng
Activity-based anorexia
POMC
Orexin
Arcuate nucleus
Male rat
Lateral hypothalamus
Activity-based anorexia alters hypothalamic POMC and orexin populations in male rats
research article
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Activity-basedAnorexiaAltersHypothalamic_2023.pdf
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text/plain
Activity-basedAnorexiaAltersHypothalamic_2023.pdf.txt