2026-04-29T23:04:19Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/193232024-09-27T07:56:12Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Fernández-Regueras, María author Carbonell, Cristina author Salete-Granado, Daniel author García, Juan-Luis author Gragera, Marcos author Pérez-Nieto, María-Ángeles author Morán-Plata, Francisco-Javier author Mayado, Andrea author Torres, Jorge-Luis author Corchete, Luis-Antonio author Usategui-Martín, Ricardo author Bueno-Martínez, Elena author Rojas-Pirela, Maura author Sabio, Guadalupe author González-Sarmiento, Rogelio author Orfao, Alberto author Laso, Francisco-Javier author Almeida, Julia author Marcos, Miguel author 2023-09-01 Excessive alcohol consumption impairs the immune system, induces oxidative stress, and triggers the activation of peripheral blood (PB) monocytes, thereby contributing to alcoholic liver disease (ALD). We analyzed the M1/M2 phenotypes of circulating classical monocytes and macrophage-derived monocytes (MDMs) in excessive alcohol drinkers (EADs). PB samples from 20 EADs and 22 healthy controls were collected for isolation of CD14+ monocytes and short-term culture with LPS/IFNγ, IL4/IL13, or without stimulation. These conditions were also used to polarize MDMs into M1, M2, or M0 phenotypes. Cytokine production was assessed in the blood and culture supernatants. M1/M2-related markers were analyzed using mRNA expression and surface marker detection. Additionally, the miRNA profile of CD14+ monocytes was analyzed. PB samples from EADs exhibited increased levels of pro-inflammatory cytokines. Following short-term culture, unstimulated blood samples from EADs showed higher levels of soluble TNF-α and IL-8, whereas monocytes expressed increased levels of surface TNF-α and elevated mRNA expression of pro-inflammatory cytokines and inducible nitric oxide synthase. MDMs from EADs showed higher levels of TNF-α and CD206 surface markers and increased IL-10 production. LPS/IFNγ induced higher mRNA expression of Nrf2 only in the controls. miRNA analysis revealed a distinctive miRNA profile that is potentially associated with liver carcinogenesis and ALD through inflammation and oxidative stress. This study confirms the predominantly pro-inflammatory profile of PB monocytes among EADs and suggests immune exhaustion features in MDMs. Antioxidants (Basel). 2023 Sep 1;12(9):1708. 10.3390/antiox12091708 2076-3921 Antioxidants (Basel, Switzerland) 37760011 http://hdl.handle.net/20.500.12105/19323 Predominantly Pro-Inflammatory Phenotype with Mixed M1/M2 Polarization of Peripheral Blood Classical Monocytes and Monocyte-Derived Macrophages among Patients with Excessive Ethanol Intake.