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oai:repisalud.isciii.es:20.500.12105/193122024-09-27T09:03:43Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Gimenez-Llorente, Daniel author Portela, Marta author Losada, Ana author Manzanares, Miguel author Andreu, Maria Jose author 2023-04-30 Hi-C studies the three-dimensional structure of the genome by detecting genome-wide chromatin regions that are in spatial proximity within the nucleus. We developed single-blastocyst Hi-C in mutant mouse embryos to genotype them on sequence. We describe steps for embryo fixation and nuclei permeabilization, after which chromatin is digested and re-ligated having incorporated a biotin-labeled nucleotide at the ligation junction. After cross-link reversal, we then detail purification of immobilized chimeric DNA ligations, library generation, sequencing, and genome-wide analysis of interactions. For complete details on the use and execution of this protocol, please refer to Andreu et al. (2022).1. STAR Protoc. 2023 Apr 30;4(2):102267. 10.1016/j.xpro.2023.102267 2666-1667 STAR protocols 37126445 http://hdl.handle.net/20.500.12105/19312 A protocol to study three-dimensional genome structure in individual mutant preimplantation mouse embryos.