2026-04-29T06:15:47Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/193092024-09-27T09:17:23Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Yadav, Upendra S
author
Biswas, Tathagata
author
Singh, Pratik N
author
Gupta, Pankaj
author
Chakraborty, Soura
author
Delgado, Irene
author
Zafar, Hamim
author
Capellini, Terence D
author
Torres, Miguel
author
Bandyopadhyay, Amitabha
funder
Ministerio de Ciencia e Innovación (España)
2024-05-08T13:51:17Z
2024-05-08T13:51:17Z
2023-07-01
Development. 2023 Jul 1;150(13):dev201335.
http://hdl.handle.net/20.500.12105/19309
37272420
10.1242/dev.201335
1477-9129
Development (Cambridge, England)
The vertebrate appendage comprises three primary segments, the stylopod, zeugopod and autopod, each separated by joints. The molecular mechanisms governing the specification of joint sites, which define segment lengths and thereby limb architecture, remain largely unknown. Existing literature suggests that reciprocal gradients of retinoic acid (RA) and fibroblast growth factor (FGF) signaling define the expression domains of the putative segment markers Meis1, Hoxa11 and Hoxa13. Barx1 is expressed in the presumptive joint sites. Our data demonstrate that RA-FGF signaling gradients define the expression domain of Barx1 in the first presumptive joint site. When misexpressed, Barx1 induces ectopic interzone-like structures, and its loss of function partially blocks interzone development. Simultaneous perturbations of RA-FGF signaling gradients result in predictable shifts of Barx1 expression domains along the proximo-distal axis and, consequently, in the formation of repositioned joints. Our data suggest that during early limb bud development in chick, Meis1 and Hoxa11 expression domains are overlapping, whereas the Barx1 expression domain resides within the Hoxa11 expression domain. However, once the interzone is formed, the expression domains are refined and the Barx1 expression domain becomes congruent with the border of these two putative segment markers.
eng
Molecular mechanism of synovial joint site specification and induction in developing vertebrate limbs.
journal article
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URL
https://repisalud.isciii.es/bitstreams/cbf11b8f-f0ff-4579-afe7-19ce47d7b642/download
File
MD5
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application/pdf
Molecular mechanism of synovial_Development_2023.pdf
URL
https://repisalud.isciii.es/bitstreams/a2c0a934-9498-4eb3-9b25-3bdc21646dab/download
File
MD5
610e41437295eecc7c66d94dbdcd37e0
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text/plain
Molecular mechanism of synovial_Development_2023.pdf.txt