2026-05-30T08:30:31Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/192902024-09-27T08:53:44Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Culemann, Stephan author Knab, Katharina author Euler, Maximilien author Wegner, Anja author Garibagaoglu, Hilal author Ackermann, Jochen author Fischer, Kim author Kienhöfer, Deborah author Crainiciuc, Georgiana author Hahn, Jonas author Grüneboom, Anika author Nimmerjahn, Falk author Uderhardt, Stefan author Hidalgo, Andres author Schett, Georg author Hoffmann, Markus H author Krönke, Gerhard author 2023-06-05 Clodronate liposomes (Clo-Lip) have been widely used to deplete mononuclear phagocytes (MoPh) to study the function of these cells in vivo. Here, we revisited the effects of Clo-Lip together with genetic models of MoPh deficiency, revealing that Clo-Lip exert their anti-inflammatory effects independent of MoPh. Notably, not only MoPh but also polymorphonuclear neutrophils (PMN) ingested Clo-Lip in vivo, which resulted in their functional arrest. Adoptive transfer of PMN, but not of MoPh, reversed the anti-inflammatory effects of Clo-Lip treatment, indicating that stunning of PMN rather than depletion of MoPh accounts for the anti-inflammatory effects of Clo-Lip in vivo. Our data highlight the need for a critical revision of the current literature on the role of MoPh in inflammation. J Exp Med. 2023 Jun 5;220(6):e20220525. 10.1084/jem.20220525 1540-9538 The Journal of experimental medicine 36976180 http://hdl.handle.net/20.500.12105/19290 Stunning of neutrophils accounts for the anti-inflammatory effects of clodronate liposomes.