2026-04-29T06:16:04Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/192862025-04-04T17:29:42Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19605col_20.500.12105_16963

00925njm 22002777a 4500 dc Esteban-Cantos, Andres author Rodríguez-Centeno, Javier author Silla-Castro, Juan Carlos author Barruz, Pilar author Sanchez-Cabo, Fatima author Saiz-Medrano, Gabriel author Nevado, Julián author Mena-Garay, Beatriz author Jiménez-González, María author de Miguel, Rosa author Bernardino, Jose Ignacio author Montejano, Rocío author Cadiñanos, Julen author Marcelo, Cristina author Gutiérrez-García, Lucía author Martínez-Martín, Patricia author Wallet, Cédrick author Raffi, François author Rodés, Berta author Arribas, José R author 2023-02 BACKGROUND: Previous epigenome-wide association studies have shown that HIV infection can disrupt the host DNA methylation landscape. However, it remains unclear how antiretroviral therapy (ART) affects the HIV-induced epigenetic modifications. METHODS: 184 individuals with HIV from the NEAT001/ANRS143 clinical trial (with pre-ART and post-ART samples [96 weeks of follow-up]) and 44 age-and-sex matched individuals without HIV were included. We compared genome-wide DNA methylation profiles in whole blood between groups adjusting for age, sex, batch effects, and DNA methylation-based estimates of leucocyte composition. FINDINGS: We identified 430 differentially methylated positions (DMPs) between HIV+ pre-ART individuals and HIV-uninfected controls. In participants with HIV, ART initiation modified the DNA methylation levels at 845 CpG positions and restored 49.3% of the changes found between HIV+ pre-ART and HIV-uninfected individuals. We only found 15 DMPs when comparing DNA methylation profiles between HIV+ post-ART individuals and participants without HIV. The Gene Ontology enrichment analysis of DMPs associated with untreated HIV infection revealed an enrichment in biological processes regulating the immune system and antiviral responses. In participants with untreated HIV infection, DNA methylation levels at top HIV-related DMPs were associated with CD4/CD8 ratios and viral loads. Changes in DNA methylation levels after ART initiation were weakly correlated with changes in CD4+ cell counts and the CD4/CD8 ratio. INTERPRETATION: Control of HIV viraemia after 96 weeks of ART initiation partly restores the host DNA methylation changes that occurred before antiretroviral treatment of HIV infection. EBioMedicine. 2023 Feb:88:104434. http://hdl.handle.net/20.500.12105/19286 36640455 10.1016/j.ebiom.2022.104434 2352-3964 EBioMedicine Effect of HIV infection and antiretroviral therapy initiation on genome-wide DNA methylation patterns.