2026-04-29T02:37:51Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/192432024-09-21T10:43:06Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Lamanna-Rama, N
author
MacDowell, K S
author
López, G
author
Leza, J C
author
Desco, M
author
Ambrosio, E
author
Soto-Montenegro, M L
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Centro de Investigación Biomédica en Red - CIBERSAM (Salud Mental)
funder
Ministerio de Sanidad (España)
funder
Fundación Alicia Koplowitz
funder
Plan Nacional de Drogas (España)
funder
Instituto de Investigación Sanitaria Gregorio Marañón
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2024-05-06T11:19:55Z
2024-05-06T11:19:55Z
2023-04
Eur Neuropsychopharmacol. 2023 Apr:69:60-76.
http://hdl.handle.net/20.500.12105/19243
36780817
10.1016/j.euroneuro.2023.01.005
1873-7862
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology
This study evaluates the long-term effects of a six and 14-week morphine withdrawal in rats pretreated with a cannabinoid agonist (CP-55,940, CP) during periadolescence. Wistar rats (33 males; 32 females) were treated with CP or its vehicle (VH) from postnatal day (PND) 28-38. At PND100, rats performed morphine self-administration (MSA, 15d/12 h/session). Eight groups were defined according to pretreatment (CP), treatment (morphine), and sex. Three [18F]FDG-PET brain images were acquired: after MSA, and after six and 14 weeks of withdrawal. PET data were analyzed with SPM12. Endocannabinoid (EC) markers were evaluated in frozen brain tissue at endpoint. Females showed a higher mean number of self-injections than males. A main Sex effect on global brain metabolism was found. FDG uptake in males was discrete, whereas females showed greater brain metabolism changes mainly in areas of the limbic system after morphine treatment. Moreover, the morphine-induced metabolic pattern in females was exacerbated when CP was previously present. In addition, the CP-Saline male group showed reduced CB1R, MAGL expression, and NAPE/FAAH ratio compared to the control group, and morphine was able to reverse CB1R and MAGL expression almost to control levels. In conclusion, females showed greater and longer-lasting metabolic changes after morphine withdrawal than males, indicating a higher vulnerability and a different sensitivity to morphine in subjects pre-exposed to CP. In contrast, males primarily showed changes in EC markers. Together, our results suggest that CP pre-exposure contributes to the modulation of brain metabolism and EC systems in a sex-dependent manner.
eng
Neuroimaging revealed long-lasting glucose metabolism changes to morphine withdrawal in rats pretreated with the cannabinoid agonist CP-55,940 during periadolescence.
journal article
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Neuroimaging revealed long-lasting_Eur Neuropsychopharmacol_2023.pdf
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Neuroimaging revealed long-lasting_Eur Neuropsychopharmacol_2023.pdf.txt