2026-05-20T23:00:59Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/191112024-11-29T17:50:43Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Boskovic, Jasminka
author
Martinez-Gago, Jaime
author
Mendez-Pertuz, Marinela
author
Buscato, Alberto
author
Martinez Torrecuadrada, Jorge Luis
author
Blasco, MA
funder
Fundacion Botin
funder
Ministerio de Economía y Competitividad (España)
2024-04-04T08:47:16Z
2024-04-04T08:47:16Z
2016-10-07
J Biol Chem . 2016 ;291(41):21829-21835.
10.1074/jbc.M116.744896
1083-351X
The Journal of biological chemistry
27563064
http://hdl.handle.net/20.500.12105/19111
Telomeres are specific DNA-protein structures found at both ends of eukaryotic chromosomes that protect the genome from degradation and from being recognized as double-stranded breaks. In vertebrates, telomeres are composed of tandem repeats of the TTAGGG sequence that are bound by a six-subunit complex called shelterin. Molecular mechanisms of telomere functions remain unknown in large part due to lack of structural data on shelterins, shelterin complex, and its interaction with the telomeric DNA repeats. TRF1 is one of the best studied shelterin components; however, the molecular architecture of the full-length protein remains unknown. We have used single-particle electron microscopy to elucidate the structure of TRF1 and its interaction with telomeric DNA sequence. Our results demonstrate that full-length TRF1 presents a molecular architecture that assists its interaction with telometic DNA and at the same time makes TRFH domains accessible to other TRF1 binding partners. Furthermore, our studies suggest hypothetical models on how other proteins as TIN2 and tankyrase contribute to regulate TRF1 function.
eng
Molecular Architecture of Full-length TRF1 Favors Its Interaction with DNA.
journal article
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URL
https://repisalud.isciii.es/bitstreams/623d5376-1752-4117-a12b-0a0071fa99c9/download
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application/pdf
moleculararquitectureoffull_2016.pdf
URL
https://repisalud.isciii.es/bitstreams/2132d0af-eb3d-4b32-a9e4-b8aa89c2089d/download
File
MD5
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text/plain
moleculararquitectureoffull_2016.pdf.txt