2026-05-16T22:07:36Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/190482024-11-14T08:49:22Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Khogali, Altayeb
author
Elnaiem, Dia-Eldin A
author
Díaz-Regañón, Ramón
author
Jibreel, Tayseer
author
Nour, Bakri Y M
author
Abdelrahman, Samira Hamid
author
Molina, Ricardo
author
Jimenez, Maribel
funder
Department for International Development (Reino Unido)
funder
Instituto de Salud Carlos III
funder
Ministry of Higher Education (Sudan)
2024-03-22T09:56:20Z
2024-03-22T09:56:20Z
2024-02-02
Trop Med Infect Dis. 2024 Feb 2;9(2):40.
10.3390/tropicalmed9020040
2414-6366
Tropical medicine and infectious disease
38393129
http://hdl.handle.net/20.500.12105/19048
A study was carried out to compare the infection rates of Leishmania donovani in Phlebotomus orientalis sandflies at different microhabitats of a VL endemic village in Gedarif state, Sudan. DNA extracts of 1078 P. orientalis sand fly females sampled by CDC light traps from indoor, outdoor, peri-domestic, and sylvatic sites, in three transmission seasons, March-June 2016-18, in Helat-Belo village, were subjected to independent PCR amplifications targeting Leishmania kDNA and the cpb gene followed by ITS1 region sequencing. Leishmania kDNA was detected in 1.4% of the 1078 P. orientalis females captured in the area. Two of these specimens showed a characteristic 741 bp band of L. donovani after cpb gene amplification. The DNA sequence of the ITS1 region of the parasites matched the ITS1 L. donovani genotype F. There were no signficant differences between rates of infection of L. donovani in P. orientalis captured at different sites. Blood meals found in infected flies origninated from human (5 specimens), cattle (4 specimens) and donkey (2 specimens). The finding of fresh cow and donkey blood in the infected flies suggests the possible role of these animals in the zoopotentiation and/or zooprophylaxis against VL. The study provides important information for VL transmission models and control programs in East Africa.
eng
Visceral leishmaniasis
Leishmania donovani
Infection rates
Phlebotomus orientalis
Sand flies
Sudan
East Africa
Infection of Leishmania donovani in Phlebotomus orientalis Sand Flies at Different Microhabitats of a Kala-Azar Endemic Village in Eastern Sudan
research article
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InfectionLeishmaniaDonovaniPhlebotomus2024.pdf
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InfectionLeishmaniaDonovaniPhlebotomus2024.pdf.txt