2026-07-21T17:02:04Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/190432024-10-31T11:44:38Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Jacqueline Forget, Camille
author
Samper-Cativiela, Clara
author
Monzon-Fernandez, Sara
author
Ugarte-Ruiz, María
author
Cuesta de la Plaza, Isabel
author
Alvarez, Julio
author
Herrera-León, Silvia
funder
Unión Europea. European Centre for Disease Prevention and Control (ECDC)
funder
Complutense University of Madrid (España)
funder
Banco Santander
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
2024-03-22T08:29:59Z
2024-03-22T08:29:59Z
2024
J Antimicrob Chemother. 2024 Apr 2;79(4):790-800.
10.1093/jac/dkae029
1460-2091
The Journal of antimicrobial chemotherapy
38366818
http://hdl.handle.net/20.500.12105/19043
Objectives: While an increase in the levels of MDR in Salmonella enterica sevorar Choleraesuis has been reported in Europe, little is known about the situation in Spain. Therefore, we first aimed to assess the phenotypic resistance profile and to determine the presence of genetic determinants of resistance of S. Choleraesuis isolates collected in animal and human. Our second objective was to identify and characterize clusters of highly related isolates. Methods: We analysed 50 human and 45 animal isolates retrieved from 2006 to 2021 using the disc diffusion method and performed WGS followed by analyses of genetic determinants and phylogenetic analysis. Results: All isolates were of ST145 and corresponded to the variant Kunzendorf. Swine isolates harboured a significantly higher number of antimicrobial resistance genes than human isolates, and often carried plasmid replicons of the IncHI2/IncHI2A type (42% of all animal isolates). In addition, we identified several MDR S. Choleraesuis strains circulating in humans and swine between 2006 and 2021. The phylogenetic analyses identified four clades associated with specific patterns of resistance genes and plasmid replicons. The clades also included isolates that differed in terms of year and region of isolation as well as host of origin. Conclusions: This One Health approach highlights that reducing human MDR S. Choleraesuis infections may require the adoption of strategies that not only seek to prevent cases in humans but also to characterize and reduce the infection burden in swine.
eng
Phenotypic and genetic characterization of antimicrobial resistance in Salmonella enterica serovar Choleraesuis isolates from humans and animals in Spain from 2006 to 2021
research article
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