2026-04-18T03:30:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/190382024-09-27T09:08:36Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Binek, Aleksandra
author
Castans, Celia
author
Jorge, Inmaculada
author
Bagwan, Navratan
author
Rodriguez, Jose Manuel
author
Fernandez-Jimenez, Rodrigo
author
Galan-Arriola, Carlos
author
Oliver, Eduardo
author
Gomez, Monica
author
Clemente-Moragón, Agustín
author
Ibáñez, Borja
author
Camafeita, Emilio
author
Vazquez, Jesus
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación ProCNIC
funder
Comunidad de Madrid (España)
funder
Fundación La Caixa
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Comisión Europea. 7 Programa Marco
funder
Ministerio de Educación, Cultura y Deporte (España)
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2024-03-21T16:39:48Z
2024-03-21T16:39:48Z
2024-01-16
Antioxidants (Basel). 2024 Jan 16;13(1):106.
2076-3921
http://hdl.handle.net/20.500.12105/19038
38247530
10.3390/antiox13010106
Antioxidants (Basel, Switzerland)
While reperfusion, or restoration of coronary blood flow in acute myocardial infarction, is a requisite for myocardial salvage, it can paradoxically induce a specific damage known as ischemia/reperfusion (I/R) injury. Our understanding of the precise pathophysiological molecular alterations leading to I/R remains limited. In this study, we conducted a comprehensive and unbiased time-course analysis of post-translational modifications (PTMs) in the post-reperfused myocardium of two different animal models (pig and mouse) and evaluated the effect of two different cardioprotective therapies (ischemic preconditioning and neutrophil depletion). In pigs, a first wave of irreversible oxidative damage was observed at the earliest reperfusion time (20 min), impacting proteins essential for cardiac contraction. A second wave, characterized by irreversible oxidation on different residues and reversible Cys oxidation, occurred at late stages (6-12 h), affecting mitochondrial, sarcomere, and inflammation-related proteins. Ischemic preconditioning mitigated the I/R damage caused by the late oxidative wave. In the mouse model, the two-phase pattern of oxidative damage was replicated, and neutrophil depletion mitigated the late wave of I/R-related damage by preventing both Cys reversible oxidation and irreversible oxidation. Altogether, these data identify protein PTMs occurring late after reperfusion as an actionable therapeutic target to reduce the impact of I/R injury.
eng
Oxidative Post-translational Protein Modifications upon Ischemia/Reperfusion Injury.
journal article
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URL
https://repisalud.isciii.es/bitstreams/feeb4b1e-a888-41dc-b3e2-09a09f48488f/download
File
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application/pdf
Oxidative Post-translational_Antioxidants_ 2024.pdf
URL
https://repisalud.isciii.es/bitstreams/c439a930-af34-4649-b930-193b4f1ee30e/download
File
MD5
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text/plain
Oxidative Post-translational_Antioxidants_ 2024.pdf.txt