2026-06-14T10:38:29Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/190362024-09-28T03:16:17Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
González-Sastre, Rosa
author
Coronel Lopez, Raquel
author
Bernabeu-Zornoza, Adela
author
Mateos-Martínez, Patricia
author
Rosca, Andreea
author
López-Alonso, Victoria
author
Liste-Noya, Isabel
funder
Agencia Estatal de Investigación (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2024-03-21T14:27:31Z
2024-03-21T14:27:31Z
2024-02
J Tissue Eng. 2024 Feb 9:15:20417314231226027.
10.1177/20417314231226027
2041-7314
Journal of tissue engineering
38343770
http://hdl.handle.net/20.500.12105/19036
Human cerebral organoids (hCOs) offer the possibility of deepening the knowledge of human brain development, as well as the pathologies that affect it. The method developed here describes the efficient generation of hCOs by going directly from two-dimensional (2D) pluripotent stem cell (PSC) cultures to three-dimensional (3D) neuroepithelial tissue, avoiding dissociation and aggregation steps. This has been achieved by subjecting 2D cultures, from the beginning of the neural induction step, to dual-SMAD inhibition in combination with CHIR99021. This is a simple and reproducible protocol in which the hCOs generated develop properly presenting proliferative ventricular zones (VZs) formed by neural precursor and radial glia (RG) that differentiate to give rise to mature neurons and glial cells. The hCOs present additional cell types such as oligodendrocyte precursors, astrocytes, microglia-like cells, and endothelial-like cells. This new approach could help to overcome some of the existing limitations in the field of organoid biotechnology, facilitating its execution in any laboratory setting.
eng
Human cerebral organoid
Pluripotent stem cells
Embryonic stem cells
Neuroepithelium
Ventricular zones
Embryoid body
Efficient generation of human cerebral organoids directly from adherent cultures of pluripotent stem cells
research article
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URL
https://repisalud.isciii.es/bitstreams/4a3a6c5b-5348-4435-a454-9c4498dff487/download
File
MD5
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application/pdf
EfficientGenerationHumanCerebralOrganoids_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/2b6c7208-518a-4ac4-9429-d1aea217bf7d/download
File
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text/plain
EfficientGenerationHumanCerebralOrganoids_2024.pdf.txt