2026-06-14T05:05:26Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/190362024-09-28T03:16:17Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617

00925njm 22002777a 4500 dc González-Sastre, Rosa author Coronel Lopez, Raquel author Bernabeu-Zornoza, Adela author Mateos-Martínez, Patricia author Rosca, Andreea author López-Alonso, Victoria author Liste-Noya, Isabel author 2024-02 Human cerebral organoids (hCOs) offer the possibility of deepening the knowledge of human brain development, as well as the pathologies that affect it. The method developed here describes the efficient generation of hCOs by going directly from two-dimensional (2D) pluripotent stem cell (PSC) cultures to three-dimensional (3D) neuroepithelial tissue, avoiding dissociation and aggregation steps. This has been achieved by subjecting 2D cultures, from the beginning of the neural induction step, to dual-SMAD inhibition in combination with CHIR99021. This is a simple and reproducible protocol in which the hCOs generated develop properly presenting proliferative ventricular zones (VZs) formed by neural precursor and radial glia (RG) that differentiate to give rise to mature neurons and glial cells. The hCOs present additional cell types such as oligodendrocyte precursors, astrocytes, microglia-like cells, and endothelial-like cells. This new approach could help to overcome some of the existing limitations in the field of organoid biotechnology, facilitating its execution in any laboratory setting. J Tissue Eng. 2024 Feb 9:15:20417314231226027. 10.1177/20417314231226027 2041-7314 Journal of tissue engineering 38343770 http://hdl.handle.net/20.500.12105/19036 Human cerebral organoid Pluripotent stem cells Embryonic stem cells Neuroepithelium Ventricular zones Embryoid body Efficient generation of human cerebral organoids directly from adherent cultures of pluripotent stem cells