2026-04-29T04:09:40Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/190342024-09-28T03:18:35Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Povedano, Eloy
author
Ruiz-Valdepeñas Montiel, Víctor
author
Sebuyoya, Ravery
author
Torrente-Rodríguez, Rebeca M
author
Garranzo-Asensio, Maria
author
Montero-Calle, Ana Maria
author
Pingarrón, José M
author
Barderas Manchado, Rodrigo
author
Bartosik, Martin
author
Campuzano, Susana
funder
Agencia Estatal de Investigación (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Health Research Council (República Checa)
funder
National Institute for Cancer Research
funder
Unión Europea. Comisión Europea. NextGenerationEU
funder
Comunidad de Madrid (España)
2024-03-21T14:16:27Z
2024-03-21T14:16:27Z
2024-03-19
Anal Chem. 2024 Mar 19;96(11):4580-4588.
http://hdl.handle.net/20.500.12105/19034
38348822
10.1021/acs.analchem.3c05474
1520-6882
Analytical chemistry
This work reports the first electrochemical bioplatforms developed for the determination of the total contents of either target miRNA or methylated target miRNA. The bioplatforms are based on the hybridization of the target miRNA with a synthetic biotinylated DNA probe, the capture of the formed DNA/miRNA heterohybrids on the surface of magnetic microcarriers, and their recognition with an antibody selective to these heterohybrids or to the N6-methyladenosine (m6A) epimark. The determination of the total or methylated target miRNA was accomplished by labeling such secondary antibodies with the horseradish peroxidase (HRP) enzyme. In both cases, amperometric transduction was performed on the surface of disposable electrodes after capturing the resulting HRP-tagged magnetic bioconjugates. Because of their increasing relevance in colorectal cancer (CRC) diagnosis and prognosis, miRNA let-7a and m6A methylation were selected. The proposed electrochemical bioplatforms showed attractive analytical and operational characteristics for the determination of the total and m6A-methylated target miRNA in less than 75 min. These bioplatforms, innovative in design and application, were applied to the analysis of total RNA samples extracted from cultured cancer cells with different metastatic profiles and from paired healthy and tumor tissues of patients diagnosed with CRC at different stages. The obtained results demonstrated, for the first time using electrochemical platforms, the potential of interrogating the target miRNA methylation level to discriminate the metastatic capacities of cancer cells and to identify tumor tissues and, in a pioneering way, the potential of the m6A methylation in miRNA let-7a to serve as a prognostic biomarker for CRC.
eng
Bringing to Light the Importance of the miRNA Methylome in Colorectal Cancer Prognosis Through Electrochemical Bioplatforms
research article
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URL
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BringingLightImportance_miRNA_2024.pdf
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Supplementary_BringingLightImportance_miRNA_2024.pdf
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BringingLightImportance_miRNA_2024.pdf.txt
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