2026-05-04T16:55:59Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/190132025-04-24T17:52:04Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16976col_20.500.12105_16974
Repisalud
author
Aranzamendi, Maitane
author
Xanthopoulou, Kyriaki
author
Sánchez-Urtaza, Sandra
author
Burgwinkel, Tessa
author
Arazo Del Pino, Rocío
author
Lucaßen, Kai
author
Perez-Vazquez, Maria
author
Oteo-Iglesias, Jesus
author
Sota, Mercedes
author
Marimón, José María
author
Seifert, Harald
author
Higgins, Paul G
author
Gallego, Lucía
funder
Basque Government (España)
funder
Biogipuzkoa Health Research Institute
2024-03-20T11:26:36Z
2024-03-20T11:26:36Z
2024-02-16
Int J Mol Sci. 2024 Feb 16;25(4):2333.
10.3390/ijms25042333
1422-0067
International journal of molecular sciences
38397011
http://hdl.handle.net/20.500.12105/19013
Infections caused by carbapenem-resistant Acinetobacter baumannii are a global threat causing a high number of fatal infections. This microorganism can also easily acquire antibiotic resistance determinants, making the treatment of infections a big challenge, and has the ability to persist in the hospital environment under a wide range of conditions. The objective of this work was to study the molecular epidemiology and genetic characteristics of two blaOXA24/40Acinetobacter baumannii outbreaks (2009 and 2020-21) at a tertiary hospital in Northern Spain. Thirty-six isolates were investigated and genotypically screened by Whole Genome Sequencing to analyse the resistome and virulome. Isolates were resistant to carbapenems, aminoglycosides and fluoroquinolones. Multi-Locus Sequence Typing analysis identified that Outbreak 1 was mainly produced by isolates belonging to ST3Pas/ST106Oxf (IC3) containing blaOXA24/40, blaOXA71 and blaADC119. Outbreak 2 isolates were exclusively ST2Pas/ST801Oxf (IC2) blaOXA24/40, blaOXA66 and blaADC30, the same genotype seen in two isolates from 2009. Virulome analysis showed that IC2 isolates contained genes for capsular polysaccharide KL32 and lipooligosacharide OCL5. A 8.9 Kb plasmid encoding the blaOXA24/40 gene was common in all isolates. The persistance over time of a virulent IC2 clone highlights the need of active surveillance to control its spread.
eng
Acinetobacter baumannii
Carbapenem resistance
Hospital outbreaks
Sequencing
Plasmid
Persistence
Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain
research article
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Supplementary_GenomicSurveillanceUncovers_2024.pdf
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