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oai:repisalud.isciii.es:20.500.12105/190132025-04-24T17:52:04Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16976col_20.500.12105_16974

00925njm 22002777a 4500 dc Aranzamendi, Maitane author Xanthopoulou, Kyriaki author Sánchez-Urtaza, Sandra author Burgwinkel, Tessa author Arazo Del Pino, Rocío author Lucaßen, Kai author Perez-Vazquez, Maria author Oteo-Iglesias, Jesus author Sota, Mercedes author Marimón, José María author Seifert, Harald author Higgins, Paul G author Gallego, Lucía author 2024-02-16 Infections caused by carbapenem-resistant Acinetobacter baumannii are a global threat causing a high number of fatal infections. This microorganism can also easily acquire antibiotic resistance determinants, making the treatment of infections a big challenge, and has the ability to persist in the hospital environment under a wide range of conditions. The objective of this work was to study the molecular epidemiology and genetic characteristics of two blaOXA24/40Acinetobacter baumannii outbreaks (2009 and 2020-21) at a tertiary hospital in Northern Spain. Thirty-six isolates were investigated and genotypically screened by Whole Genome Sequencing to analyse the resistome and virulome. Isolates were resistant to carbapenems, aminoglycosides and fluoroquinolones. Multi-Locus Sequence Typing analysis identified that Outbreak 1 was mainly produced by isolates belonging to ST3Pas/ST106Oxf (IC3) containing blaOXA24/40, blaOXA71 and blaADC119. Outbreak 2 isolates were exclusively ST2Pas/ST801Oxf (IC2) blaOXA24/40, blaOXA66 and blaADC30, the same genotype seen in two isolates from 2009. Virulome analysis showed that IC2 isolates contained genes for capsular polysaccharide KL32 and lipooligosacharide OCL5. A 8.9 Kb plasmid encoding the blaOXA24/40 gene was common in all isolates. The persistance over time of a virulent IC2 clone highlights the need of active surveillance to control its spread. Int J Mol Sci. 2024 Feb 16;25(4):2333. 10.3390/ijms25042333 1422-0067 International journal of molecular sciences 38397011 http://hdl.handle.net/20.500.12105/19013 Acinetobacter baumannii Carbapenem resistance Hospital outbreaks Sequencing Plasmid Persistence Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain