2026-05-22T17:22:33Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/190092025-04-24T06:04:11Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Velasco, Carlos
author
Ortega, Javier
author
Ricón, Jaime
author
Romero, Beatriz
author
Juan Ferre, Lucia de
author
Domínguez, Lucas
author
Dominguez-Rodriguez, Mercedes
author
Moreno, Inmaculada
author
Álvarez, Julio
author
Bezos, Javier
funder
Unión Europea. Comisión Europea. H2020. ERA-NET
funder
Ministerio de Ciencia e Innovación (España)
funder
Ministerio de Agricultura, Pesca y Alimentación (España)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
2024-03-19T14:44:30Z
2024-03-19T14:44:30Z
2024-02
Front Vet Sci. 2024 Feb 8:11:1358413.
10.3389/fvets.2024.1358413
2297-1769
Frontiers in veterinary science
38389583
http://hdl.handle.net/20.500.12105/19009
Caprine tuberculosis (TB) is a zoonotic disease caused by members of the Mycobacterium tuberculosis complex. TB eradication programs in goats are based on the single and comparative intradermal tuberculin tests (SITT and CITT, respectively). Antibody-based diagnostic techniques have emerged as potential diagnostic tools for TB. P22 ELISA has been previously evaluated using samples collected after the intradermal tuberculin tests to maximize the sensitivity, a phenomenon known as booster effect. However, there is no information available on whether the use of this diagnostic strategy could lead to a decrease of its specificity (Sp). The aim of the present study was to elucidate the interference effect of a recent CITT on the Sp of the P22 ELISA in serum and milk samples collected at different times after the CITT from a TB-free herd (n = 113). The number of reactors to P22 ELISA was significantly higher (p < 0.01) on serum samples collected 15 days post-CITT compared to day 0, showing a decrease in Sp from 99.1% (95% CI; 95.2-99.8%) to 88.5% (95% CI; 81.3-93.2%). The number of reactors and the quantitative values of P22 ELISA were significantly higher (p < 0.01) in serum samples compared to milk. No significant (p > 0.05) changes in the Sp of the P22 ELISA were observed throughout the different time samplings using milk No significant (p > 0.05) changes were observed on days 30 and 60 post-CITT. In conclusion, the booster effect strategy may significantly decrease the Sp of P22 ELISA in TB-free herds when serum samples are used but not when milk is tested.
eng
Caprine tuberculosis
Intradermal tuberculin test
P22 ELISA
Booster effect
Specificity
Effect of a recent intradermal test on the specificity of P22 ELISA for the diagnosis of caprine tuberculosis
research article
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URL
https://repisalud.isciii.es/bitstreams/15ee986d-2b53-452a-b715-b12662b952af/download
File
MD5
85691b1bce02b1842ae11083f685ba87
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application/pdf
EffectRecentIntradermalTest_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/a7aab108-9baf-4516-b90c-72051d13c76e/download
File
MD5
5f2a44e562f99e7e0085ca8318d33450
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text/plain
EffectRecentIntradermalTest_2024.pdf.txt