2026-04-29T22:57:41Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/190052024-11-29T11:18:03Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16982
Repisalud
author
Matas-Méndez, Pablo
author
Ávalos, Gabriel
author
Caballero-Gómez, Javier
author
Dashti, Alejandro
author
Castro-Scholten, Sabrina
author
Jiménez-Martín, Débora
author
Gonzalez-Barrio, David
author
Muñoz-de-Mier, Gemma J
author
Bailo-Barroso, Begoña
author
Cano-Terriza, David
author
Mateo, Marta
author
Nájera, Fernando
author
Xiao, Lihua
author
Köster, Pamela Carolina
author
García-Bocanegra, Ignacio
author
Carmena, David
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Comisión Europea. NextGenerationEU
funder
Alfonso X el Sabio University (España)
funder
Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Regional Government of Andalusia (España)
funder
Regional Government of Castile-La Mancha (España)
2024-03-19T10:51:30Z
2024-03-19T10:51:30Z
2024-01-22
Animals (Basel). 2024 Jan 22;14(2):340.
10.3390/ani14020340
2076-2615
Animals : an open access journal from MDPI
38275800
http://hdl.handle.net/20.500.12105/19005
Cryptosporidium spp. and Giardia duodenalis are the main non-viral causes of diarrhoea in humans and domestic animals globally. Comparatively, much less information is currently available in free-ranging carnivore species in general and in the endangered Iberian lynx (Lynx pardinus) in particular. Cryptosporidium spp. and G. duodenalis were investigated with molecular (PCR and Sanger sequencing) methods in individual faecal DNA samples of free-ranging and captive Iberian lynxes from the main population nuclei in Spain. Overall, Cryptosporidium spp. and G. duodenalis were detected in 2.4% (6/251) and 27.9% (70/251) of the animals examined, respectively. Positive animals to at least one of them were detected in each of the analysed population nuclei. The analysis of partial ssu rRNA gene sequences revealed the presence of rodent-adapted C. alticolis (n = 1) and C. occultus (n = 1), leporid-adapted C. cuniculus (n = 2), and zoonotic C. parvum (n = 2) within Cryptosporidium, and zoonotic assemblages A (n = 5) and B (n = 3) within G. duodenalis. Subgenotyping analyses allowed for the identification of genotype VaA19 in C. cuniculus (gp60 locus) and sub-assemblages AI and BIII/BIV in G. duodenalis (gdh, bg, and tpi loci). This study represents the first molecular description of Cryptosporidium spp. and G. duodenalis in the Iberian lynx in Spain. The presence of rodent/leporid-adapted Cryptosporidium species in the surveyed animals suggests spurious infections associated to the Iberian lynx's diet. The Iberian lynx seems a suitable host for zoonotic genetic variants of Cryptosporidium (C. parvum) and G. duodenalis (assemblages A and B), although the potential risk of human transmission is regarded as limited due to light parasite burdens and suspected low excretion of infective (oo)cysts to the environment by infected animals. More research should be conducted to ascertain the true impact of these protozoan parasites in the health status of the endangered Iberian lynx.
eng
Epidemiology
Transmission
Public health
Zoonosis
PCR
Genotyping
ssu rRNA
gdh
bg
tpi
Detection and Molecular Diversity of Cryptosporidium spp. and Giardia duodenalis in the Endangered Iberian Lynx (Lynx pardinus), Spain
research article
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