2026-04-29T22:57:34Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/189602024-11-29T16:32:15Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Delgado-Chaves, Fernando M author Martínez-García, Pedro Manuel author Herrero-Ruiz, Andrés author Gómez-Vela, Francisco author Divina, Federico author Jimeno-González, Silvia author Cortes-Ledesma, Felipe author 2022-10 Type II DNA topoisomerases relax topological stress by transiently gating DNA passage in a controlled cut-and-reseal mechanism that affects both DNA strands. Therefore, they are essential to overcome topological problems associated with DNA metabolism. Their aberrant activity results in the generation of DNA double-strand breaks, which can seriously compromise cell survival and genome integrity. Here, we profile the transcriptome of human-telomerase-immortalized retinal pigment epithelial 1 (RPE-1) cells when treated with merbarone, a drug that catalytically inhibits type II DNA topoisomerases. We performed RNA-Seq after 4 and 8 h of merbarone treatment and compared transcriptional profiles versus untreated samples. We report raw sequencing data together with lists of gene counts and differentially expressed genes. Data Brief . 2022 Aug 1:44:108499. 10.1016/j.dib.2022.108499 2352-3409 Data in brief 35983130 http://hdl.handle.net/20.500.12105/18960 Data of transcriptional effects of the merbarone-mediated inhibition of TOP2.