2026-04-29T06:16:13Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/189152024-09-27T09:32:35Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Plata-Gómez, Ana Belén
author
Crespo, María
author
de la Calle Arregui, Celia
author
de Prado-Rivas, Lucía
author
Sabio, Guadalupe
author
Efeyan, Alejo
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Agencia Estatal de Investigación (España)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
2024-03-11T12:06:07Z
2024-03-11T12:06:07Z
2021-12-17
STAR Protoc. 2021 Oct 29;2(4):100918.
http://hdl.handle.net/20.500.12105/18915
34755120
10.1016/j.xpro.2021.100918
2666-1667
STAR protocols
We present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yield, viability, and modulation of the mTOR pathway. This protocol can be adapted to other signaling cascades and is compatible with multiple readouts. For complete details on the use and execution of this protocol, please refer to Ortega-Molina et al. (2021).
eng
Protocol for the assessment of mTOR activity in mouse primary hepatocytes.
journal article
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URL
https://repisalud.isciii.es/bitstreams/d5875de7-32de-4371-aae2-e7cd1e73b15a/download
File
MD5
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application/pdf
Protocol for the assessment_STAR Protoc_2021.pdf
URL
https://repisalud.isciii.es/bitstreams/7e622cf5-5b62-4352-85cf-9a3dc400e0ba/download
File
MD5
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text/plain
Protocol for the assessment_STAR Protoc_2021.pdf.txt