2026-04-29T06:16:06Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/189152024-09-27T09:32:35Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Plata-Gómez, Ana Belén author Crespo, María author de la Calle Arregui, Celia author de Prado-Rivas, Lucía author Sabio, Guadalupe author Efeyan, Alejo author 2021-12-17 We present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yield, viability, and modulation of the mTOR pathway. This protocol can be adapted to other signaling cascades and is compatible with multiple readouts. For complete details on the use and execution of this protocol, please refer to Ortega-Molina et al. (2021). STAR Protoc. 2021 Oct 29;2(4):100918. http://hdl.handle.net/20.500.12105/18915 34755120 10.1016/j.xpro.2021.100918 2666-1667 STAR protocols Protocol for the assessment of mTOR activity in mouse primary hepatocytes.