2026-08-29T09:17:23Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/189132024-09-27T08:49:19Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Vega-Pérez, Adrián author Villarrubia, Laura H author Godio, Cristina author Gutiérrez-González, Alejandra author Feo-Lucas, Lidia author Ferriz, Margarita author Martínez-Puente, Natalia author Alcaín, Julieta author Mora, Alfonso author Sabio, Guadalupe author López-Bravo, María author Ardavín, Carlos author Villarrubia, Laura H. author 2021-11-09 Peritoneal immune cells reside unanchored within the peritoneal fluid in homeostasis. Here, we examined the mechanisms that control bacterial infection in the peritoneum using a mouse model of abdominal sepsis following intraperitoneal Escherichia coli infection. Whole-mount immunofluorescence and confocal microscopy of the peritoneal wall and omentum revealed that large peritoneal macrophages (LPMs) rapidly cleared bacteria and adhered to the mesothelium, forming multilayered cellular aggregates composed by sequentially recruited LPMs, B1 cells, neutrophils, and monocyte-derived cells (moCs). The formation of resident macrophage aggregates (resMφ-aggregates) required LPMs and thrombin-dependent fibrin polymerization. E. coli infection triggered LPM pyroptosis and release of inflammatory mediators. Resolution of these potentially inflammatory aggregates required LPM-mediated recruitment of moCs, which were essential for fibrinolysis-mediated resMφ-aggregate disaggregation and the prevention of peritoneal overt inflammation. Thus, resMφ-aggregates provide a physical scaffold that enables the efficient control of peritoneal infection, with implications for antimicrobial immunity in other body cavities, such as the pleural cavity or brain ventricles. Immunity. 2021 Nov 9;54(11):2578-2594.e5. 10.1016/j.immuni.2021.10.007 1097-4180 1074-7613 Immunity 34717795 http://hdl.handle.net/20.500.12105/18913 Resident macrophage-dependent immune cell scaffolds drive anti-bacterial defense in the peritoneal cavity.