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oai:repisalud.isciii.es:20.500.12105/188812024-11-28T15:24:04Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Mascaraque, Ainhoa author Kowalczyk, Wioleta author Fernández, Tahia author Palomares, Francisca author Mayorga, Cristobalina author Andreu, David author Rojo, Javier author 2015-10 Mannosylation facilitates uptake and internalization of immunogenic peptides by antigen-processing cells expressing mannose receptors at their surface, such as DC-SIGN, a lectin that plays a key role in the immune response against different pathogens. This internalization, processing and subsequent MHC presentation may result in a strong T cell stimulation. Here, we hypothesized that combining mannose glycodendrons with multivalent presentation of peptide epitopes in a likewise dendron format would yield hybrid constructs, named glycodendropeptides (GDPs), with the capacity to enhance peptide immunogenicity, hence providing a novel and versatile platform for applications in immunotherapy. Thus, GDPs of different valencies displaying the NP366-374 epitope, a conserved sequence from the influenza A virus nucleoprotein (NP), have been built by two click chemistry-based methodologies and assessed as potential flu vaccine candidates. Preliminary evaluation of the ability of these constructs to stimulate dendritic cell maturation and lymphocyte proliferation was promising, showing the highest-functionalized NP366-374 GDPs as inducing the strongest immunostimulatory effect. 2040-2511 http://hdl.handle.net/10668/2274 http://hdl.handle.net/20.500.12105/18881 10.1039/C5MD00133A 2040-2503 MedChemComm Moléculas de adhesión celular Química clic Secuencia conservada Dendrómeros Células dendróticas Epítopos Inmunoterapia Lectinas tipo C Manosa Lectinas de unión a manosa Péptidos Proteínas de unión al ARN Receptores de superficie celular Linfocitos T Vacunas Proteínas del centro vírico Glycodendropeptides stimulate dendritic cell maturation and T cell proliferation: a potential influenza A virus immunotherapy