2026-05-16T22:07:41Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/187632024-09-21T22:04:55Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Nuñez, Rafael author Rodriguez, Maria Jose author Palomares, Francisca author Gomez, Francisca author Jabato, Fernando M author Cordoba-Caballero, Jose author Seoane, Pedro author Losada, Jorge author Rojo, Javier author Torres, Maria Jose author Perkins, James Richard author Mayorga, Cristobalina author 2022-02-18 To investigate food allergy-tolerance mechanisms induced through allergen-specific immunotherapy we used RNA-Sequencing to measure gene expression in lymph-node-derived dendritic cells from Pru p 3-anaphylactic mice after immunotherapy with glycodendropeptides at 2 nM and 5 nM, leading to permanent tolerance and short-term desensitization, respectively. Gene expression was also measured in mice receiving no immunotherapy (anaphylaxis); and in which anaphylaxis could never occur (antigen-only). Compared to anaphylaxis, the antigen-only group showed the greatest number of expression-changes (411), followed by tolerant (186) and desensitized (119). Only 29 genes changed in all groups, including Il12b, Cebpb and Ifngr1. The desensitized group showed enrichment for genes related to chronic inflammatory response, secretory granule, and regulation of interleukin-12 production; the tolerant group showed genes related to cytokine receptor activity and glucocorticoid receptor binding, suggesting distinct pathways for similar outcomes. We identified genes and processes potentially involved in the restoration of long-term tolerance via allergen-specific immunotherapy, representing potential prognostic biomarkers. 10.1038/s41598-022-06186-8 2045-2322 Scientific reports http://hdl.handle.net/10668/19583 35181694 http://hdl.handle.net/20.500.12105/18763 Transcriptional changes in dendritic cells underlying allergen specific induced tolerance in a mouse model.