2026-06-14T02:28:43Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/185972024-02-27T14:58:05Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author Ribeiro, Marcelo C author Rivera-Arbeláez, José M author Cofiño-Fabres, Carla author Schwach, Verena author Slaats, Rolf H author Ten Den, Simone A author Vermeul, Kim author van den Berg, Albert author Pérez-Pomares, José M author Segerink, Loes I author Guadix, Juan A author Passier, Robert 2024-02-27T14:58:05Z 2024-02-27T14:58:05Z 2022-02-04 10.3390/jpm12020214 2075-4426 Journal of personalized medicine http://hdl.handle.net/10668/21372 35207702 http://hdl.handle.net/20.500.12105/18597 Cardiomyocytes derived from human pluripotent stem cells (hPSC-CMs) hold a great potential as human in vitro models for studying heart disease and for drug safety screening. Nevertheless, their associated immaturity relative to the adult myocardium limits their utility in cardiac research. In this study, we describe the development of a platform for generating three-dimensional engineered heart tissues (EHTs) from hPSC-CMs for the measurement of force while under mechanical and electrical stimulation. The modular and versatile EHT platform presented here allows for the formation of three tissues per well in a 12-well plate format, resulting in 36 tissues per plate. We compared the functional performance of EHTs and their histology in three different media and demonstrated that tissues cultured and maintained in maturation medium, containing triiodothyronine (T3), dexamethasone, and insulin-like growth factor-1 (TDI), resulted in a higher force of contraction, sarcomeric organization and alignment, and a higher and lower inotropic response to isoproterenol and nifedipine, respectively. Moreover, in this study, we highlight the importance of integrating a serum-free maturation medium in the EHT platform, making it a suitable tool for cardiovascular research, disease modeling, and preclinical drug testing. eng cardiac performance contractile force engineered heart tissues hPSC-CMs serum-free versatile platform A New Versatile Platform for Assessment of Improved Cardiac Performance in Human-Engineered Heart Tissues. research article