2026-06-14T03:46:30Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/185272024-02-19T15:32:35Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Caballano-Infantes, Estefanía author García-García, Alberto author Lopez-Gomez, Carlos author Cueto, Alejandro author Robles-Diaz, Mercedes author Ortega-Alonso, Aida author Martín-Reyes, Flores author Alvarez-Alvarez, Ismael author Arranz-Salas, Isabel author Ruiz-Cabello, Francisco author Lucena, Isabel M author García-Fuentes, Eduardo author Andrade, Raúl J author García-Cortes, Miren author 2021-12-28 Non-alcoholic fatty liver disease (NAFLD) and idiosyncratic drug-induced liver injury (DILI) could share molecular mechanisms involving the immune system. We aimed to identify activation immunological biomarkers in invariant natural killer T (iNKT) and CD4/CD8+ T cells in NAFLD and DILI. We analyzed the activation profile (CD69, CD25, and HLA-DR) and natural killer group 2 member D (NKG2D) on iNKT cells, and CD4/CD8 T cells in peripheral blood mononuclear cells from NAFLD, with or without significant liver fibrosis, and DILI patients. There was an increase in iNKT cells in NAFLD patients compared to DILI or control subjects. Regarding the cellular activation profile, NAFLD with significant liver fibrosis (F ≥ 2) displayed higher levels of CD69+iNKT cells compared to NAFLD with none or mild liver fibrosis (F ≤ 1) and control patients. CD69+iNKT positively correlated with insulin resistance, aspartate aminotransferase (AST) level, liver fibrosis-4 index (FIB4) and AST to Platelet Ratio Index (APRI). DILI patients showed an increase in CD69+ and HLA-DR+ in both CD4+ and CD8+ T cells, detecting the most relevant difference in the case of CD69+CD8+ T cells. CD69+iNKT may be a biomarker to assess liver fibrosis progression in NAFLD. CD69+CD8+ T cells were identified as a potential distinctive biomarker for distinguishing DILI from NAFLD. 10.3390/biomedicines10010055 2227-9059 Biomedicines http://hdl.handle.net/10668/20811 35052736 http://hdl.handle.net/20.500.12105/18527 drug-induced liver injury (DILI) immune response immunophenotype liver fibrosis non-alcoholic fatty liver disease (NAFLD) Differential iNKT and T Cells Activation in Non-Alcoholic Fatty Liver Disease and Drug-Induced Liver Injury.