2026-05-21T23:03:05Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/185062024-11-28T14:47:36Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Santiago-Fernández, Concepción author Martín-Reyes, Flores author Tome, Monica author Gutierrez-Repiso, Carolina author Fernandez-Garcia, Diego author Ocaña-Wilhelmi, Luis author Rivas-Becerra, Jose author Tatzber, Franz author Pursch, Edith author Tinahones, Francisco J. author García-Fuentes, Eduardo author Garrido-Sánchez, Lourdes author 2021-11-18 Background: Little is known about the effects of hypoxia on scavenger receptors (SRs) levels in adipocytes. We analyzed the effect of morbid obesity and hypoxia on SRs and inflammation markers in human visceral adipocytes and whether ox-LDL modify the inflammatory profile produced by hypoxia. Methods: We studied in 17 non-obese and 20 subjects with morbid obesity (MO) the mRNA expression of HIF-1α, SRs (LOX-1, MSR1, CL-P1 and CXCL16), IL6 and TNFα in visceral adipocytes and the effect of hypoxia with or without ox-LDL on visceral in vitro-differentiated adipocytes (VDA). Results: HIF-1α, TNFα, IL6, LOX-1, MSR1 and CXCL16 expression in adipocytes was increased in MO when compared with those in non-obese subjects (p < 0.05). The expression of most of the inflammatory markers and SRs gene correlated with HIF-1α. In VDA, hypoxia increased TNFα, IL6, MSR1, CXCL16 and CL-P1 (p < 0.05) in non-obese subjects, and TNFα, IL6, MSR1 and CXCL16 (p < 0.05) in MO. Silencing HIF-1α prevented the increase of TNFα, IL6, LOX-1, MSR1, CL-P1 and CXCL16 expression (p < 0.05). The combination of hypoxia and ox-LDL produced higher TNFα expression (p = 0.041). Conclusions: Morbid obesity and hypoxia increased SRs and inflammatory markers in visceral adipocytes. In a hypoxic state, ox-LDL increased the proinflammatory response of visceral adipocytes to hypoxia. 10.3390/biomedicines9111715 2227-9059 Biomedicines http://hdl.handle.net/10668/4456 34829944 http://hdl.handle.net/20.500.12105/18506 Hypoxia Scavenger receptors Oxidized LDL Adipocytes Morbid obesity Hipoxia Receptores depuradores Receptores de LDL oxidadas Adipocitos Obesidad mórbida Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia