2026-04-29T04:09:47Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/183782024-11-28T15:17:28Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Teuwen, Laure-Anne author De Rooij, Laura P. M. H. author Cuypers, Anne author Rohlenova, Katerina author Dumas, Sébastien J. author García-Caballero, Melissa author Meta, Elda author Amersfoort, Jacob author Taverna, Federico author Becker, Lisa M. author Veiga, Nuphar author Cantelmo, Anna Rita author Geldhof, Vincent author Conchinha, Nadine V. author Kalucka, Joanna author Treps, Lucas author Conradi, Lena-Christin author Khan, Shawez author Karakach, Tobias K. author Soenen, Stefaan author Vinckier, Stefan author Schoonjans, Luc author Eelen, Guy author Van Laere, Steven author Dewerchin, Mieke author Dirix, Luc author Mazzone, Massimiliano author Luo, Yonglun author Vermeulen, Peter author Carmeliet, Peter author 2021-06-15 Tumor vessel co-option is poorly understood, yet it is a resistance mechanism against anti-angiogenic therapy (AAT). The heterogeneity of co-opted endothelial cells (ECs) and pericytes, co-opting cancer and myeloid cells in tumors growing via vessel co-option, has not been investigated at the single-cell level. Here, we use a murine AAT-resistant lung tumor model, in which VEGF-targeting induces vessel co-option for continued growth. Single-cell RNA sequencing (scRNA-seq) of 31,964 cells reveals, unexpectedly, a largely similar transcriptome of co-opted tumor ECs (TECs) and pericytes as their healthy counterparts. Notably, we identify cell types that might contribute to vessel co-option, i.e., an invasive cancer-cell subtype, possibly assisted by a matrix-remodeling macrophage population, and another M1-like macrophage subtype, possibly involved in keeping or rendering vascular cells quiescent. http://hdl.handle.net/10668/3614 http://hdl.handle.net/20.500.12105/18378 34133923 10.1016/j.celrep.2021.109253 2211-1247 Cell Reports Tumor vessel co-option Tumor angiogenesis Anti-angiogenic therapy Metastasis Resistance Single-cell RNA sequencing Endothelial cells Pericytes Macrophages Cancer cells Inductores de la angiogénesis Metástasis de la neoplasia Células endoteliales Pericitos Macrófagos Tumor vessel co-option probed by single-cell analysis