2026-05-22T23:34:12Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/183762024-11-28T14:52:34Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author Martínez-de la Puente, Josué author Gutiérrez-López, Rafael author Díez-Fernández, Alazne author Soriguer, Ramón C. author Moreno-Indias, Isabel author Figuerola, Jordi authoraffiliation [Martínez-de la Puente,J; Gutiérrez-López,R; Díez-Fernández,A; Soriguer,RC; Figuerola,J] Estación Biológica de Doñana (EBD-CSIC), Seville, Spain. [Martínez-de la Puente,J; Soriguer,RC; Figuerola,J] Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain. [Moreno-Indias,I] Department of Endocrinology and Nutrition, Virgen de la Victoria University Hospital, Instituto de Investigación Biomédica de Málaga (IBIMA), University of Malaga, Málaga, Spain. [Moreno-Indias,I] Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Madrid, Spain. 2024-02-19T15:29:13Z 2024-02-19T15:29:13Z 2021-06-13 10.3389/fmicb.2020.562220 1664-302X Frontiers in Microbiology http://hdl.handle.net/10668/3797 33519724 http://hdl.handle.net/20.500.12105/18376 Both intrinsic and extrinsic factors affect the capacity of mosquitoes for the transmission of vector-borne pathogens. Among them, mosquito microbiota may play a key role determining the development of pathogens in mosquitoes and the cost of infections. Here, we used a wild avian malaria-mosquito assemblage model to experimentally test the role of vector microbiota on the cost of infection and their consequences for parasite development. To do so, a cohort of Culex pipiens mosquitoes were treated with antibiotics, including gentamicin sulfate and penicillin-streptomycin, to alter their microbiota, and other cohort was treated with sterilized water as controls. Subsequently, both cohorts were allowed to feed on Plasmodium infected or uninfected house sparrows (Passer domesticus). The antibiotic treatment significantly increased the survival rate of mosquitoes fed on infected birds while this was not the case of mosquitoes fed on uninfected birds. Additionally, a higher prevalence of Plasmodium in the saliva of mosquitoes was found in antibiotic treated mosquitoes than in mosquitoes of the control group at 20 days post exposure (dpe). Analyses of the microbiota of a subsample of mosquitoes at 20 dpe suggest that although the microbiota diversity did not differ between individuals of the two treatments, microbiota in control mosquitoes had a higher number of unique features and enriched in biochemical pathways related to the immune system than antibiotic treated ones. In sum, this study provides support for the role of mosquito microbiota on mosquito survival and the presence of parasite DNA in their saliva. eng Culex pipiens Ecology-diseases Malaria Microbiome Parasite transmission Vector-borne pathogens Virulence Microbiota Parásitos Virulencia Culex Parasitología Enfermedades transmitidas por vectores Mosquitos vectores Effects of Mosquito Microbiota on the Survival Cost and Development Success of Avian Plasmodium research article