2026-07-21T21:48:41Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/183332024-11-28T15:39:28Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Antequera-Gómez, María Luisa author Díaz-Martínez, Luis author Guadix, Juan Antonio author Sánchez-Tévar, Ana María author Sopeña-Torres, Sara author Hierrezuelo, Jesús author Doan, Hung K. author Leveau, Johan H. J. author deVicente, Antonio author Romero, Diego author 2021-05-06 Bacillus cereus is a common food-borne pathogen that is responsible for important outbreaks of food poisoning in humans. Diseases caused by B. cereus usually exhibit two major symptoms, emetic or diarrheic, depending on the toxins produced. It is assumed that after the ingestion of contaminated vegetables or processed food, spores of enterotoxigenic B. cereus reach the intestine, where they germinate and produce the enterotoxins that are responsible for food poisoning. In our study, we observed that sporulation is required for the survival of B. cereus in leaves but is dispensable in ready-to-eat vegetables, such as endives. We demonstrate that vegetative cells of B. cereus that are originally impaired in sporulation but not biofilm formation are able to reach the intestine and cause severe disorders in a murine model. Furthermore, our findings emphasise that the number of food poisoning cases associated with B. cereus is underestimated and suggest the need to revise the detection protocols, which are based primarily on spores and toxins. 10.1111/1751-7915.13816 1751-7915 Microbial biotechnology http://hdl.handle.net/10668/3933 33955675 http://hdl.handle.net/20.500.12105/18333 Bacillus cereus Emetics Enterotoxins Spores Food poisoning Eméticos Enterotoxinas Esporas Enfermedades transmitidas por los alimentos Sporulation is dispensable for the vegetable-associated life cycle of the human pathogen Bacillus cereus