2026-06-14T03:31:15Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/183112024-11-28T14:53:49Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Naren, Gaowa
author
Larsson, Wera
author
Benitez-Martin, Carlos
author
Li, Shiming
author
Pérez-Inestrosa, Ezequiel
author
Albinsson, Bo
author
Andréasson, Joakim
authoraffiliation
[Naren,G; Larsson,W; Li,S; Albinsson,B; Andréasson,J] Chemistry and Chemical Engineering, Chemistry and Biochemistry, Chalmers University of Technology, Göteborg, Sweden. [Benitez-Martin,C; Pérez-Inestrosa,E] Universidad de Malaga-IBIMA, Departamento de Química Organica, E-29071 Málaga, Spain. [Benitez-Martin,C; Pérez-Inestrosa,E] Centro Andaluz de Nanomedicina y Biotecnología (BIONAND), Malaga, Spain.
2024-02-19T15:26:52Z
2024-02-19T15:26:52Z
2021-04-15
10.1039/d1sc01071a
2041-6539
Chemical Science
http://hdl.handle.net/10668/4165
34123335
http://hdl.handle.net/20.500.12105/18311
A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.
eng
Fluorescence
Microscopy, fluorescence
Fluorescent dyes
UV
Fluorescencia
Microscopía fluorescente
Colorantes fluorescentes
Rayos ultravioleta
Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
research article