2026-06-14T03:44:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/182922025-05-05T14:44:34Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Caro, Carlos
author
Gámez, Francisco
author
Quaresma, Pedro
author
Páez-Muñoz, Jose María
author
Domínguez, Alejandro
author
Pearson, John R
author
Pernía Leal, Manuel
author
Beltrán, Ana M.
author
Fernandez-Afonso, Yilian
author
De la Fuente, Jesús M.
author
Franco, Ricardo
author
Pereira, Eulália
author
García-Martín, Maria Luisa
authoraffiliation
[Caro,C; Páez-Muñoz,JM; Domínguez,A; Pearson,JR; García-Martín,ML] BIONAND—Centro Andaluz de Nanomedicina y Biotecnología (Junta de Andalucía-Universidad de Málaga), Málaga, Spain. [Gámez,F] Departamento de Química Física, Universidad de Granada, Granada, Spain. [Quaresma,P: Pereira,E] REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Porto, Portugal. [Pernía Leal,M] Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad de Sevilla, Seville, Spain. [Beltrán,AM] Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Escuela Politécnica Superior, Universidad de Sevilla, Sevilla, Spain. [Fernandez-Afonso,Y; De la Fuente,JM] Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, Zaragoza, Spain. [De la Fuente,JM; García-Martín,ML] Biomedical Research Networking Center in Bioengineering, Biomaterials &Nanomedicine (CIBER-BBN), Madrid, Spain [Franco,R] UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal.
2024-02-19T15:26:37Z
2024-02-19T15:26:37Z
2021-03-20
10.3390/pharmaceutics13030416
1999-4923
Pharmaceutics
http://hdl.handle.net/10668/4488
33804636
http://hdl.handle.net/20.500.12105/18292
In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X-ray computed tomography (CT). Additionally, due to the presence of the local surface plasmon resonances of gold, the NPs showed exploitable "light-to-heat" conversion ability in the near-infrared (NIR) region, a key attribute for effective photothermal therapies (PTT). In vitro experiments revealed biocompatibility as well as excellent efficiency in killing glioblastoma cells via PTT. The in vivo nontoxicity of the NPs was demonstrated using zebrafish embryos as an intermediate step between cells and rodent models. To warrant that an effective therapeutic dose was achieved inside the tumor, both intratumoral and intravenous routes were screened in rodent models by MRI and CT. The pharmacokinetics and biodistribution confirmed the multimodal imaging CA capabilities of the Fe@AuNPs and revealed constraints of the intravenous route for tumor targeting, dictating intratumoral administration for therapeutic applications. Finally, Fe@Au NPs were successfully used for an in vivo proof of concept of imaging-guided focused PTT against glioblastoma multiforme in a mouse model.
eng
Imaging-guided therapy
Multimodal imaging
Contrast agent
MRI
CT
Photothermal therapy
Zebrafish
Temperature
Tissues
Nanoparticles
Imagen multimodal
Medios de contraste
Imagen por resonancia magnética
TomografíacComputarizada por rayos X
Terapia fototérmica
Pez cebra
Temperatura
Tejidos
Nanopartículas
Neoplasias
Fe3O4-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy
research article