2026-06-14T03:51:13Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/182922025-05-05T14:44:34Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Caro, Carlos author Gámez, Francisco author Quaresma, Pedro author Páez-Muñoz, Jose María author Domínguez, Alejandro author Pearson, John R author Pernía Leal, Manuel author Beltrán, Ana M. author Fernandez-Afonso, Yilian author De la Fuente, Jesús M. author Franco, Ricardo author Pereira, Eulália author García-Martín, Maria Luisa author 2021-03-20 In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X-ray computed tomography (CT). Additionally, due to the presence of the local surface plasmon resonances of gold, the NPs showed exploitable "light-to-heat" conversion ability in the near-infrared (NIR) region, a key attribute for effective photothermal therapies (PTT). In vitro experiments revealed biocompatibility as well as excellent efficiency in killing glioblastoma cells via PTT. The in vivo nontoxicity of the NPs was demonstrated using zebrafish embryos as an intermediate step between cells and rodent models. To warrant that an effective therapeutic dose was achieved inside the tumor, both intratumoral and intravenous routes were screened in rodent models by MRI and CT. The pharmacokinetics and biodistribution confirmed the multimodal imaging CA capabilities of the Fe@AuNPs and revealed constraints of the intravenous route for tumor targeting, dictating intratumoral administration for therapeutic applications. Finally, Fe@Au NPs were successfully used for an in vivo proof of concept of imaging-guided focused PTT against glioblastoma multiforme in a mouse model. 10.3390/pharmaceutics13030416 1999-4923 Pharmaceutics http://hdl.handle.net/10668/4488 33804636 http://hdl.handle.net/20.500.12105/18292 Imaging-guided therapy Multimodal imaging Contrast agent MRI CT Photothermal therapy Zebrafish Temperature Tissues Nanoparticles Imagen multimodal Medios de contraste Imagen por resonancia magnética TomografíacComputarizada por rayos X Terapia fototérmica Pez cebra Temperatura Tejidos Nanopartículas Neoplasias Fe3O4-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy