2026-04-29T20:47:17Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/181832025-05-29T18:44:20Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Salazar-Roa, María
author
Trakala, Marianna
author
Álvarez-Fernández, Mónica
author
Valdés-Mora, Fátima
author
Zhong, Cuiqing
author
Muñoz, Jaime
author
Yu, Yang
author
Peters, Timothy J
author
Graña Castro, Osvaldo
author
Serrano, Rosa
author
Zapatero-Solana, Elisabet
author
Abad, María
author
Bueno, María José
author
Gómez de Cedrón, Marta
author
Fernández-Piqueras, José
author
Serrano, Manuel
author
Blasco, MA
author
Wang, Da-Zhi
author
Clark, Susan J
author
Izpisua-Belmonte, Juan Carlos
author
Ortega Jimenez, Sagrario
author
Malumbres, Marcos
funder
Asociación Española Contra el Cáncer
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Fundación La Caixa
funder
Fundación Ramón Areces
funder
Comunidad de Madrid (España)
funder
National Breast Cancer Foundation (Australia)
funder
National Health and Medical Research Council (Australia)
funder
Worldwide Cancer Research
funder
Fundación Banco Santander
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
funder
Cure Cancer Australia
2024-02-13T09:32:04Z
2024-02-13T09:32:04Z
2020-08-17
EMBO J . 2020;39(16):e104324.
10.15252/embj.2019104324
1460-2075
The EMBO journal
32614092
http://hdl.handle.net/20.500.12105/18183
Full differentiation potential along with self-renewal capacity is a major property of pluripotent stem cells (PSCs). However, the differentiation capacity frequently decreases during expansion of PSCs in vitro. We show here that transient exposure to a single microRNA, expressed at early stages during normal development, improves the differentiation capacity of already-established murine and human PSCs. Short exposure to miR-203 in PSCs (miPSCs) induces a transient expression of 2C markers that later results in expanded differentiation potency to multiple lineages, as well as improved efficiency in tetraploid complementation and human-mouse interspecies chimerism assays. Mechanistically, these effects are at least partially mediated by direct repression of de novo DNA methyltransferases Dnmt3a and Dnmt3b, leading to transient and reversible erasure of DNA methylation. These data support the use of transient exposure to miR-203 as a versatile method to reset the epigenetic memory in PSCs, and improve their effectiveness in regenerative medicine.
eng
Transient exposure to miR-203 enhances the differentiation capacity of established pluripotent stem cells.
journal article
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TransientexposuretomiR-203_2020.pdf.txt