2026-08-29T09:25:01Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/181782024-11-28T15:18:19Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Villatoro, Antonio J.
author
Alcoholado, Cristina
author
Martín-Astorga, María del Carmen
author
Rico, Gustavo
author
Fernández, Viviana
author
Becerra, José
authoraffiliation
[Villatoro,AJ; Alcoholado,C; Martín-Astorga,MC, Rico,G; Becerra,J] Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, University of Málaga, IBIMA, Málaga, Spain. [Villatoro,AJ; Fernández,V] Instituto de Immunología Clínica y Terapia Celular (IMMUNESTEM), Málaga, Spain. [Alcoholado,C] Biomedicine Research Institute of Malaga (IBIMA), Campus Universitario Teatinos, Málaga, Spain. [Alcoholado,C; Rico,G; Becerra,J] Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, (CIBER-BBN), Malaga, Spain. [Rico,G; Becerra,J] Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, Málaga, Spain.
2024-02-12T19:48:23Z
2024-02-12T19:48:23Z
2020-12-29
10.1371/journal.pone.0244327
1932-6203
PloS One
http://hdl.handle.net/10668/3504
33373367
http://hdl.handle.net/20.500.12105/18178
Limbal stem cells (LSCs) are a quiescent cell population responsible for the renewal of the corneal epithelium. Their deficiency is responsible for the conjunctivization of the cornea that is seen in different ocular pathologies, both in humans and in the canine species. The canine species represents an interesting preclinical animal model in ocular surface pathologies. However, the role of LSCs in physiological and pathological conditions in canine species is not well understood. Our objective was to characterize for the first time the soluble factors and the proteomic profile of the secretome and exosomes of canine LSCs (cLSCs). In addition, given the important role that fibroblasts play in the repair of the ocular surface, we evaluated the influence of the secretome and exosomes of cLSCs on their proliferation in vitro. Our results demonstrated a secretory profile of cLSCs with high concentrations of MCP-1, IL-8, VEGF-A, and IL-10, as well as significant production of exosomes. Regarding the proteomic profile, 646 total proteins in the secretome and 356 in exosomes were involved in different biological processes. Functionally, the cLSC secretome showed an inhibitory effect on the proliferation of fibroblasts in vitro, which the exosomes did not. These results open the door to new studies on the possible use of the cLSC secretome or some of its components to treat certain pathologies of the ocular surface in canine species.
eng
Dogs
Stem cells
Epithelium, corneal
Fibroblasts
Exosomes
Corneal diseases
Perros
Células madre
Epitelio corneal
Fibroblastos
Exosomas
Enfermedades de la cornea
Characterization of the secretory profile and exosomes of limbal stem cells in the canine species
research article