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oai:repisalud.isciii.es:20.500.12105/181782024-11-28T15:18:19Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Villatoro, Antonio J. author Alcoholado, Cristina author Martín-Astorga, María del Carmen author Rico, Gustavo author Fernández, Viviana author Becerra, José author 2020-12-29 Limbal stem cells (LSCs) are a quiescent cell population responsible for the renewal of the corneal epithelium. Their deficiency is responsible for the conjunctivization of the cornea that is seen in different ocular pathologies, both in humans and in the canine species. The canine species represents an interesting preclinical animal model in ocular surface pathologies. However, the role of LSCs in physiological and pathological conditions in canine species is not well understood. Our objective was to characterize for the first time the soluble factors and the proteomic profile of the secretome and exosomes of canine LSCs (cLSCs). In addition, given the important role that fibroblasts play in the repair of the ocular surface, we evaluated the influence of the secretome and exosomes of cLSCs on their proliferation in vitro. Our results demonstrated a secretory profile of cLSCs with high concentrations of MCP-1, IL-8, VEGF-A, and IL-10, as well as significant production of exosomes. Regarding the proteomic profile, 646 total proteins in the secretome and 356 in exosomes were involved in different biological processes. Functionally, the cLSC secretome showed an inhibitory effect on the proliferation of fibroblasts in vitro, which the exosomes did not. These results open the door to new studies on the possible use of the cLSC secretome or some of its components to treat certain pathologies of the ocular surface in canine species. 10.1371/journal.pone.0244327 1932-6203 PloS One http://hdl.handle.net/10668/3504 33373367 http://hdl.handle.net/20.500.12105/18178 Dogs Stem cells Epithelium, corneal Fibroblasts Exosomes Corneal diseases Perros Células madre Epitelio corneal Fibroblastos Exosomas Enfermedades de la cornea Characterization of the secretory profile and exosomes of limbal stem cells in the canine species