2026-04-29T15:03:56Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/181752024-11-28T14:42:51Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Guerrero-Cózar, Israel author Perez-Garcia, Cathaysa author Benzekri, Hicham author Sánchez, J.J. author Seoane, Pedro author Cruz, Fernando author Gut, Marta author Zamorano, Maria Jesus author Claros, M. Gonzalo author Manchado, Manuel author 2020-12-14 The Senegalese sole (Solea senegalensis) is an economically important flatfish species. In this study, a genome draft was analyzed to identify microsatellite (SSR) markers for whole-genome genotyping. A subset of 224 contigs containing SSRs were preselected and validated by using a de novo female hybrid assembly. Overall, the SSR density in the genome was 886.7 markers per megabase of genomic sequences and the dinucleotide motif was the most abundant (52.4%). In silico comparison identified a set of 108 SSRs (with di-, tetra- or pentanucleotide motifs) widely distributed in the genome and suitable for primer design. A total of 106 markers were structured in thirteen multiplex PCR assays (with up to 10-plex) and the amplification conditions were optimized with a high-quality score. Main genetic diversity statistics and genotyping reliability were assessed. A subset of 40 high polymorphic markers were selected to optimize four supermultiplex PCRs (with up to 11-plex) for pedigree analysis. Theoretical exclusion probabilities and real parentage allocation tests using parent-offspring information confirmed their robustness and effectiveness for parental assignment. These new SSR markers were combined with previously published SSRs (in total 229 makers) to construct a new and improved integrated genetic map containing 21 linkage groups that matched with the expected number of chromosomes. Synteny analysis with respect to C. semilaevis provided new clues on chromosome evolution in flatfish and the formation of metacentric and submetacentric chromosomes in Senegalese sole. 10.1038/s41598-020-78397-w 2045-2322 Scientific Reports http://hdl.handle.net/10668/3584 33318526 http://hdl.handle.net/20.500.12105/18175 Chromosome Mapping Female Flatfishes Genetic variation Genome Multiplex polymerase chain reaction Pedigree Synteny Mapeo cromosómico Femenino Peces planos Variación genética Genoma Reacción en cadena de la polimerasa multiplex Linaje Sintenía Development of whole-genome multiplex assays and construction of an integrated genetic map using SSR markers in Senegalese sole