2026-05-22T07:34:24Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/181442024-11-28T14:42:52Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Villatoro, Antonio J.
author
Martín-Astorga, María del Carmen
author
Alcoholado, Cristina
author
Becerra, José
authoraffiliation
[Villatoro,AJ; Martín-Astorga,MC; Alcoholado,C; Becerra,J] Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, University of Málaga, IBIMA, Málaga, Spain. [Villatoro,AJ] Instituto de Immunología Clínica y Terapia Celular (IMMUNESTEM), Málaga, Spain. [Alcoholado,C; Becerra,J] Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain. [Becerra,J] Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, Málaga, Spain.
2024-02-12T19:47:45Z
2024-02-12T19:47:45Z
2020-11-02
10.1186/s12917-020-02623-w
1746-6148
BMC Veterinary Research
http://hdl.handle.net/10668/3600
33138803
http://hdl.handle.net/20.500.12105/18144
Background: Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results: A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-shaped morphology and well-defined round vesicles. The size distribution of exosomes was between 37 and 140 nm, and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosome cargo. We also found that exosomes modified the proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Additionally, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture. Conclusions: Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed higher potential in adipose tissue development and neurogenesis, while cBM-MSC production was associated with immunity, cell mobilization and haematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating their fundamental role in the development and maturation of dogs in the early stages of their life.
eng
Canine colostrum milk
Mesenchymal stem cells
Exosomes
Dog
Anti-oxidative capacity
Calostro
Perros
Células madre mesenquimatosas
Exosomas
Antioxidantes
Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity
research article