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oai:repisalud.isciii.es:20.500.12105/181032024-11-28T15:21:24Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author Pericuesta, Eva author Laguna-Barraza, Ricardo author Ramos-Ibeas, Priscila author Gutierrez-Arroyo, Julia L. author Navarro, Juan A. author Vera, Katia author Sanjuan, Carlos author Baixeras, Elena author Rodríguez de Fonseca, Fernando author Gutierrez-Adan, Alfonso authoraffiliation [Pericuesta,E; Laguna-Barraza,R; Ramos-Ibeas,P; Gutierrez-Arroyo,JL; Gutierrez-Adan,A] Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain. [Navarro,JA; Vera,K; Rodriguez de Fonseca,F] Laboratorio de Neuropsicofarmacología, Unidad de Gestión Clínica de Salud Mental, Instituto IBIMA, Hospital Regional Universitario de Málaga, Málaga, Spain. [Sanjuan,C] Euronutra S.L., Málaga, Spain. [Baixeras,E] Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Málaga, Málaga, Spain. 2024-02-12T19:47:04Z 2024-02-12T19:47:04Z 2020-08-22 http://hdl.handle.net/10668/3863 http://hdl.handle.net/20.500.12105/18103 32842637 10.3390/ijms21176049 1422-0067 International Journal of Molecular Sciences Polycystic ovarian syndrome (PCOS) is the main cause of female infertility. It is a multifactorial disorder with varying clinical manifestations including metabolic/endocrine abnormalities, hyperandrogenism, and ovarian cysts, among other conditions. D-Chiro-inositol (DCI) is the main treatment available for PCOS in humans. To address some of the mechanisms of this complex disorder and its treatment, this study examines the effect of DCI on reproduction during the development of different PCOS-associated phenotypes in aged females and two mouse models of PCOS. Aged females (8 months old) were treated or not (control) with DCI for 2 months. PCOS models were generated by treatment with dihydrotestosterone (DHT) on Days 16, 17, and 18 of gestation, or by testosterone propionate (TP) treatment on the first day of life. At two months of age, PCOS mice were treated with DCI for 2 months and their reproductive parameters analyzed. No effects of DCI treatment were produced on body weight or ovary/body weight ratio. However, treatment reduced the number of follicles with an atretic cyst-like appearance and improved embryo development in the PCOS models, and also increased implantation rates in both aged and PCOS mice. DCI modified the expression of genes related to oocyte quality, oxidative stress, and luteal sufficiency in cumulus-oocyte complexes (COCs) obtained from the aged and PCOS models. Further, the phosphorylation of AKT, a main metabolic sensor activated by insulin in the liver, was enhanced only in the DHT group, which was the only PCOS model showing glucose intolerance and AKT dephosphorylation. The effect of DCI in the TP model seemed mediated by its influence on oxidative stress and follicle insufficiency. Our results indicate that DCI works in preclinical models of PCOS and offer insight into its mechanism of action when used to treat this infertility-associated syndrome. eng Polycystic ovary syndrome D-chiro-inositol Oocyte Embryo Fertility Oxidative stress Glucose intolerance Síndrome del ovario poliquístico Oocitos Estructuras embrionarias Fertilidad Estrés oxidativo Intolerancia a la glucosa D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome research article